We narrowed to 161 results for: Arch-GFP

... lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9 and further...2016 was an exciting year for genome engineering research. A variety of new tools came out including the...

...from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  ...collection of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent...sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g...to either the N-or the C-terminus. Even if a researcher’s gene of interest isn’t in the ORF collection...in the Davidson lab for the last four years. “Researchers can make their own target if it is not already...can essentially cut and paste quite quickly. Researchers should keep in mind that a very simple sequential...deposit the tools he and his team created with the research community at large, "[Fluorescent proteins are...

...either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set...genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process...genome tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't...

...blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common...is a component of the immune system useful as a research reagent? Let’s find out! What is an antibody? ...system and a tool that can be used for a variety of research applications. Within the immune system, antibodies...ability of antibodies to bind proteins is useful for research applications as well because they allow scientists...and initiates different immune cascades. In the research setting, antibodies of different isotypes can ...antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to spot the whole...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...

...containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA...viral titre to the number of cells transduced by a GFP encoded virus, and the reverse transcriptase activity...increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically...inefficient at infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike...than the first one and can be used routinely in a research laboratory. The risk of generating RCL is low ...technique, there’s always a small chance that a researcher could infect him or herself with the oncogenic... generation lentiviral vectors can be used for research and for clinical purposes, however improving the...

...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple...expand the toolbox for researchers looking to study norepinephrine, the Patriarchi lab continues to work...sensors was developed, named GRABNE, which helped researchers visualize norepinephrine (Feng et al., 2019).... and were only available as green probes. The Patriarchi Lab aimed to overcome these limitations by developing...nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References...Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for...

...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs ...biomedical research". The family entered the biomedical field’s spotlight after researchers discovered...animals have impacted humans through biomedical research. The Camelid family is composed of camels, llamas... llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller forms of ...experiments, due to their simple structure    sdAbs in research These advantages have contributed to sdAbs becoming...variable domains (aka nanomice), which gives researchers the “best of both worlds” - a familiar lab model...

...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ...Fluorophores In this post, Luke Lavis from Janelia Research Campus reminds us that, although fluorescent proteins.... To learn how you can use minigenomes in your research, check out this post! R Bodies: Membrane-Rupturing... post, Jessica Polka, formerly a postdoctoral researcher in the Silver Lab at Harvard University, describes...Genome Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits...In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab, describes how you can better...Addgene Blog useful resources for the biological research community! ...

...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...collaborative endeavor providing plasmids to researchers in developing countries. We are proud to introduce...this important agricultural plant. Her current research focuses on endophytes, bacteria, and fungi that...applying gene technology to bacteria and plants. Her research involves cloning cry genes from the soil bacterium...groups of scientists working in a wide range of research fields and geographical locations. We are excited...

...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome ...also bring benefits to researchers conducting basic or early pre-clinical research. However, standard terms...concept of gRNA design is still challenging for researchers using CRISPR, and that standardizing and improving...resources and publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say...

...endocytosis of a fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference...performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively...believe that these results will be beneficial to the research community.    So, why are we so excited? For starters...inventory and more time providing new materials to the research community. References 1. Coroadinha, A. S., ...

...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...found in bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature... Produce synthetic viruses that can be used in research or for therapeutics Addgene has compiled various...

...Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...     HcKCRs represent a long-sought tool for researchers to control K+ gradients and can be used as a ...of the most commonly used protein tags - a quick search for ‘Myc tag’ in the Addgene catalog pulled up ...

...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...no completely genetic tools that would allow researchers to label just a fraction of a single genetically-defined...

..., hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI... Molecule of the Year. Additionally, in 2014 researchers at MD Anderson Cancer Center presented results...

...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...restricting the availability of Cre, lox sites lets researchers control the spatial and temporal activation of...

...collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available...our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors... testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes. This...recently released these controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad...broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression...interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter...mCherry in the absence of Cre, and expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium ...

...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...Cpf1.   Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct...from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147. PubMed PMID: 25122746. PubMed... Our Guide Pages Find CRISPR Plasmids for Your Research Browse Our CRISPR Multiplexing Resources ...

...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, all...Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group at UCSF. It’s been only a few months since... more than 20 labs to form the QBI Coronavirus Research Group (QCRG) to do just this.  In under a month...with requests to share our plasmids with other researchers. We have been excited to see so much interest...problem more quickly. Figure 2: Getting our research in line, with a row of FedEx envelopes waiting...shared spreadsheet and it takes time away from our research. For all these reasons, the QCRG is delighted ...requesting plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with...

...fluorescent protein topics page Get the basics on GFP Find microbiology blog posts Resources on Addgene.org...development by openly sharing their resources with the research community. To honor their legacy, we launched ...choice to share their fluorescent protein based research. This year, we put out another call for applications...genetic variants, what does that mean? Previous research shows that approximately 75% of all protein-coding...variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry site and...a variant has wild-type levels of abundance, the eGFP will be stable and fluoresce. But if the variant...unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing the library are then...