... members (like me!) are dedicated to creating a public and accessible database for low-cost, high-quality...positive, it will be released through the website for public reference. The Gift of Giving As an open-source...for goldilocks antibodies and, alongside that, a publicly available 2D/3D mouse atlas from antibodies that...resolving the antibody crisis. The mAb3D Atlas is a public service to the science community, and I am excited...

...Institute (Harvard University), we developed two new enabling technologies for the field of synthetic biology... journal Cell just six weeks later. The Zika publication timeline I think we can speak for all the authors...that can take pathogen sequence information from public databases to the production of validated sensors...PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold...

...to dimerization (i.e. EGFP) [1, 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem ...and difficult to ignore [1, 2]. 2. Fusion to an obligate dimer or oligomer Several cell proteins normally...even if they were described as monomeric in a publication. The gold standard in assays used to determine...moderately dimerizing FPs such as EGFP, but not for obligate oligomers such as DsRed or high affinity FPs such...

...highlight some of the reagents that have been made publicly available through Addgene.  What makes CRISPR... Individual gRNAs are designed in silico using publicly available gRNA design software and synthesized...derivatives of Cas9. Several CRISPR libraries are publicly available through Addgene, with more being added...information on CRISPR screens check out Addgene’s 2016 publication “Practical Considerations for Using Pooled Lentiviral...

... far beyond the laboratory and seriously damage public trust in scientists, experiments involving potential...negligible level. One major reason we chose to publish the concept and likely capabilities of RNA-guided...biology and genetics because the underlying advance enabling RNA-guided gene drives is Cas9 genome editing....about gene drives can be found in the referenced publications. Thank you to our guest blogger! An inventor...

...autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly...disease that results in combined deafness and blindness) is caused by defects in the gene MYO7a, at ~7...further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the...restores vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID: 11326284...

...papers. Maybe they started their deposit prior to publication and were able to include specific plasmid ID ... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...terms we can use to find materials from this publication. Searching for the title of a paper works You...filters Not every plasmid search will start from a publication. You may have an idea for a plasmid that would...

...PubMed PMID: 26422227. Find plasmids from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex...PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik, et al. Genome-wide...PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver, Benjamin P., et al....PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated...

...path.  A part of the career path story for most established grant professionals is learning to succeed in... with clear budget narratives, logic models, established partnerships and collaborations, and commitments...grants profession? The number of organizations, both public and private, that qualify for grants, the amount... panels and to review education materials for publishers. When the community orchestra needed a grant ...

...sequence assembly of the zebrafish genome was published in 2013, revealing that about 70% of human genes...for generating transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust...zygote transforms into an organism with a clearly established body plan including head, eyes, and tail. Compared...different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality...

...Principal Investigator meeting of the NSF EDGE (Enabling Discovery Through Genomic Tools) Program and I... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because...

...have the same blind spots, and there wouldn’t be anyone to question these blind spots. Blind spots often...result of our diversity which in turn minimizes the blind spots in our work.  Agile at Addgene We follow an...

... do need multiple first/corresponding author publications in quality journals. It helps to come from the...a job. The currency of scientific success is publications and grants. 2. Write succinct and easy to understand...what I was proposing. The result of my test was humbling. My conviction that I had a flair for communication.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi...and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W,...

...Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo...capsid, while Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids... plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing (Chen et al., 2023). AAV-BI30, developed...Caltech’s CLOVER Center’s extensive repository of published systemic AAV experiments and Addgene’s AAV Data...Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species...

...are reusable, saving time and money on future assemblies. If you are new to MoClo, give our MoClo 101 ...save time and money on studies that require many assemblies or that compare the effects of different DNA ...PLoS ONE, edited by Jean Peccoud, vol. 6, no. 2, Public Library of Science (PLoS), 18 Feb. 2011, p. e16765...PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science (PLoS), 30 May 2018, p. e0197185...

... with another lab, in another field maybe, and publish Join a lab that has multiple collaborations, even... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend... for Researchers called Vitae. Vitae recently published a career development framework for career skills...

... about intellectual property rights, pending publications, and commercial use often come up. An MTA can...trials Acknowledgement in recipient’s future publications relating to the material Limited liability...applications on modifications or inventions Right to publish without editorial comment or review by the Provider...

...ask direct questions for specific feedback in non-public settings. Ask over coffee.  Have regular 1:1 meetings...information to make change whenever possible. Be public about changes influenced by feedback. If you don't...Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein...