We narrowed to 364 results for: DUR
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TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....normal! Action Items: Start a career journal during your current job and note what makes your heart...writing and presenting for non-scientist audiences during your time in graduate school through outreach organizations...programs can connect you with alumni before and during your application process so you can learn more!...
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A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m....minimize recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019)... showing the mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous... Some classes of neurons transiently express TH during development or have low levels of TH mRNA without... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m....world of academic research can be quite daunting. During my first year of college, I knew I wanted to try... provided me with much-needed emotional support during my journey. When you are hunting for labs to join... and that you want to travel in new directions. During my grad school interviews, one of the professors... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog PostPublishedMarch 14, 2017, 2:30 p.m....environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells over-express...trials going amiss and patient deaths have occurred during CAR-T cell tests because treatment regimens were... likely to be positively impacted by the trial. During clinical trials, expansion of participants based... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....to double-stranded, and finally, drop once more during a final temperature increase. If there is a single.... Flipping your experimental and control values during analysis can allow you to see this fold change ... as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process. Sci Rep 8, 8716 (2018... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....ourselves accosted by dozens of tiny black dots during our stay there. Yet capturing the flies is nearly...everywhere find and re-contain any specimens that escape during routine lab activities. Here, we demonstrate the... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog PostPublishedNov. 15, 2016, 3:30 p.m.... of medical school. 7:05 - 10:55 : Alex's focus during graduate school - DNA damage and longevity. 10:...repair informed his decision to work with CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute ... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog PostPublishedDec. 16, 2014, 8:01 p.m....just over a year old, and it has grown steadily during 2014. We were excited to have more and more scientists...Margo R. Monroe Best wishes for your research during this holiday season and we look forward to seeing... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog PostPublishedNov. 25, 2014, 4:02 p.m....coactivator induced in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog PostPublishedAug. 13, 2020, 1:21 p.m....sciences, I knew I would run into some challenges during my internship, but nothing could have prepared ...challenges would be from doing a remote internship during a pandemic. Before I knew how bad COVID was getting... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog PostPublishedNov. 27, 2013, 2:54 p.m.... from the software folks and started Hack Days. During Hack Days, teams at Addgene will forgo their regular...website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success at ... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m....academic scientists. Expanding plasmid sharing during COVID-19 Realizing this limitation, we worked closely...setting. In light of the need for basic research during the current pandemic, universities are showing ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m.... the importance of leaving room for serendipity during your PhD studies and offer two simple steps to ...capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....brain slices Visualization of DA release dynamics during locomotion in freely moving mice Record DA dynamics... in live mice Report modulation of DA signaling during learning and reward consumption in live mice Find... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog PostPublishedJune 4, 2020, 1:22 p.m....chromosome. One of the main causes of degradation during plasmid DNA extraction and purification from E....though in their capacity to maintain DNA stability during storage over prolonged periods of time, especially... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....technical hurdle. ChIP-seq requires cross-linking during an initial fixation step. This serves to stabilize...transcriptional regulatory elements is controlled during development using the Drosophila wing as a model... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m.... project. Dissemination of the knowledge gained during this period occurs infrequently and rarely makes... wondered how we could make the insights gained during this time more visible and thereby have a positive... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....enterprise or that there weren’t some pretty hefty bumps during my graduate training. I particularly think that...that I thought about careers outside of academia during my graduate training. PhDs have many roles to play... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...t new to Addgene, we are excited to share the procedures and Python scripts with laboratories and viral...