We narrowed to 859 results for: ELL

...to the well (either directly or by binding to an antibody that is coating the bottom of the well), and ...coating the wells to capture antigen from a sample, whereas antigen is bound directly to the wells in a direct... the wells are typically already coated with the antibody. Then, you add your sample to the wells, and...sample are bound to the wells of a plate instead of an antibody. Coating the wells with a purified protein...ELISA well As mentioned above, the measurement of the concentration of proteins in an ELISA well is based...the reaction, turns the solution yellow. The absorbance of each well, which is measured using a microplate... are used to detect proteins within a 96- or 384-well, flat-bottomed plate. The basic principle of an ...

...contaminating your transduction with the packaging cells. Packaging cell debris can be removed either by filtration...-500 g). After removing packaging cell debris, virus can be pelleted by ultracentrifugation (75,000 - ...ultracentrifugation, virus may appear as a white pellet. The pellet can then be rinsed and resuspended in cold... you can reduce the volume of cell culture medium on the packaging cells immediately following transfection...efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and... the packaging cells after 1–3 days of selection. A lower percentage of surviving cells may be the result...adeno-associated virus transduction of cells." American Journal of Physiology-Cell Physiology 291.2 (2006): C386...

...restoring mCherry expression. Cells can then be sorted using Fluorescence-activated cell sorting (FACS) and the...easily determined by comparing cells expressing only eGFP expression to cells expressing eGFP and mCherry...expressed in cells. mCherry is constitutively expressed to identify successfully transfected cells. C-to-T ...eGFP and mCherry positive cells by the number of only mCherry positive cells (Martin et al., 2019). ...TREE) to purify single base edited cells without the need for single cell isolation and downstream sequencing...fluorescence and cell sorting using FACS which could limit their use across different cell types and systems...sgRNA targeting the GFPACG to the cells. As expected, only the cells that contained the cytidine base ...

... carried by extracellular vesicles (EVs), edited transcripts could endow neighboring cells with pro-oncogenic...observe degradation as well as endocytic trafficking in real time through extracellular-specific and full ...kinase essential for cell proliferation signaling. CRISPR knock-in HiBiT-tagged EGFR cells allowed researchers...Degradation of Misfolded CFTR. Cells, 12(23), 2741. https://doi.org/10.3390/cells12232741  Kamio, T., Kono, Y.,... fusions utilizing bulky tags can disrupt normal cell biology and make it hard to capture true protein...researchers with a means to quickly iterate, work across cell models, and even introduce mutations. This vector-based...and other fluorescent proteins revolutionized live-cell protein detection, their bulk (~27 kDa) can perturb...

...be different for each transduced cell, there’s no telling which cells may harbor these unwanted interruptions...purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing... chromosome of the cell. This integrated viral genome is then part of the cellular DNA… forever!  This...viral entry into the host cell.  Transfecting these three plasmids into cells enables the production of...lost through cycles of cell division, though the rate of loss greatly depends on cell turnover. Why use ...expression in non-dividing cells IDLVs can also be useful for transducing non-dividing cells, as they avoid the... transduced cells and quantifying the integrated viral DNA along with a reference cellular gene. When ...

...Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells Note:...intervening proteins, lipid bilayers, organelles or other cellular impediments. FRET has been used in many...endogenous biomolecules in cells nonspecifically, and they cannot cross cell membranes, so they are best... ion channels, or cell surface receptors) or with experiments on permeabilized cells. Biarsenical labeling...these compounds cross cell membranes, thus enabling FRET measurements in intact cells. The Gradia Lab has...-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized cells  Cy5NTAc ...-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized cells  Cy5NTAc ...

...Non-Dividing Cells Transient Gene Expression Low Tropism for Mouse Cells and Hematopoietic Cells Low Packaging...cargo” to target cells and organisms, and when that cargo is designed to make you sick…well, you feel it....fuses with the target cell’s membrane, allowing the viral particle access into the cell. The viral envelope... of cell the virus will target and fuse with. In nature, this determines which organisms and cells they...viruses can be engineered to be cell type specific. Once inside the host cell, the virus unleashes its cargo...wide range of cell types that are typically ‘difficult to transfect’, such as primary cells. They can also...Viruses as biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral...

...Calcium phosphate Cells Transformed cell lines Transformed cell lines Transformed cell lines Cargo Nucleic...identify and enrich positive cells or a selection marker to generate stable cell lines. Transient or stable...and enrich positive cells. Likely contains a selection marker to generate stable cell lines. Packaging and...wide variety of mammalian cell lines. Useful for difficult to transfect cell types and can be used in ...protein are degraded within the cell.   Delivery methods for mammalian cell lines As mentioned above, the...components into your target cells. Delivery of these components into mammalian cell lines is quite broad and...Electroporation / Nucleofection Microinjection Cells Many types Single cells Cargo Nucleic acids or protein Nucleic...

...With regard to target cells or organism, some primary cells and differentiated cell types are picky when...packaging plasmids into a viral production cell line, usually HEK-293 cells. When all of these viral plasmids ...secreted into the cellular media, making viral collection fairly easy. Producer cell media can be collected... rogue HEK-293 cell). This virus-containing media can then be placed on whatever cells you would like ...just zapping your cells? That really depends on two things. First, what type of cell or organism context...viruses as well. Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with ...process of transferring foreign nucleic acid into a cell by a viral agent. Transductions are similar to transfections...

...prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells. The... continuous cellular processes (e.g., protein synthesis), while amp only affects the cell wall synthesis...got amp? Well, amp is less stable than its counterparts and is also known to produce satellite colonies...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418...Check icon" %} Spectinomycin Excellent No Just bacteria Not SHuffle cells {% icon icon_set="fontawesome...icon" %} Zeocin Excellent No Bacteria, plants, and eukaryotes Not Tn5-containing cells {% icon icon_set..., penicillin, and is a beta-lactam that inhibits cell wall synthesis. The AmpR (amp resistance) gene produces...

...Winslow’s lab to label cells after cell-cell interactions. Using G-baToN, “sender” cells express GFP on the... and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, GFP is...the receiver cell, and the receiver cell turns green. This system can be used in many cell types including...engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron system....brgcm3sw     New system developed to track cell-cell interactions By Jennifer Tsang GFP-based Touching...including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic...of the interaction between the sender cell and the receiver cell. Image from Tang et al., 2020.   ...

... of cell types in the nervous system. While certain cell types, such as principal excitatory cells and...neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected promoters for cell types...great performance in cellular functional imaging and in protein fusions. The Gadella lab has deposited all...with edited “pools” of cells, eliminating the time-consuming step of single-cell isolation. Agudelo et ...gene. Koo M, et al. Cell Systems. 2016. PubMed PMID: 28189581 Peters J, et al. Cell. 2016. PubMed PMID:...generation Deriving iPSCs (induced pluripotent stem cells) through the expression of transcription factors...the established model for reprogramming somatic cells. A common method for generating iPSCs to express...

...therapy and ex vivo cell engineering applications and is especially well-suited for lung cells via airway administration...epithelial cells, liver, CNS (limited penetration), retina, hematopoietic cells, and immune cells. B) AAV11...Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing stem cell heterogeneity dictates clonal...the acquisition of leukemic driver mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org/10.1016...you'll find in this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery of TnpB...plant genome editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging...connections with ATLAS Bright and photostable yellow fluorescent protein mGold2 By Mike Lacy Get your...

...PV-positive cells out of all BiPVe3-dTom cells. C) Confocal stack of a biocytin-filled cell expressing ...particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected...brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as...Scheme for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1...contains everything needed to replicate inside a host cell but with space to insert your genetic cargo of choice...promoter drives robust gene expression in mammalian cells. Flexible tagging: Choose untagged or add a versatile...stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular by design: Key elements...

...lets AAVs “unlock” and infect specific cells. This protein shell packages the vector’s genetic material...particle sneaks its way into cells. Generally, AAV serotypes have a primary cellular receptor and sometimes ..., 2006). AAV6 is particularly well-suited for transduction of lung cells via airway administration (Halbert...has tropism for cardiac and liver cells (Issa et al., 2023), as well as skeletal muscle, although muscle...transduction efficiency, and is an excellent choice for targeting cardiac cells (Bish et al., 2008). It can ...-associated viruses. Cell, S0092-8674(25)00692-0. https://doi.org/10.1016/j.cell.2025.06.026 Halbert, ... Therapy: An Overview. Cells, 12(5), 785. https://doi.org/10.3390/cells12050785 Kaludov, N., Brown, K....

...linkage of intra- and extracellular environments. Found on nearly every cell in the human body, integrins are...array of processes, including cell adhesion, migration, and signaling, as well as immune response, tissue... that protrude from the cell membrane, physically connecting the extracellular environment and the cytoskeleton...ligand binding, mediating cell adhesion and migration, initiating intracellular signal cascades, and allowing... the extracellular matrix. During immune responses, this same conformation encourages immune cell adhesion...tensions before they would enable cells to spread, resulting in cells that elongated and morphed asymmetrically...on the BJ-5ta fibroblast cells, integrin αVβ1 was critical in initiating cell spreading and two other ...

... is technically cell-permeable, its use in live cells can sometimes result in cellular toxicity due to...its propensity to bind cellular components. Some dyes target specific organelles like mitochondria, and... Dyes that can pass through cell membranes can prove useful for live-cell imaging. Experimental context...Additionally, cell permeability is a critical factor, as it influences the dye's ability to enter cells without...enabled the visualization of both organic tissues and cell cultures, opening biological interiors to many inquisitive... dye or ligand to make your selection easier, as well as highlight some recent advancements within this...collection contains many self-labeling protein fusions as well as empty backbones to clone your own protein of ...

...matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer,...Making contact with a cell Although an individual AAV particle’s ability to infect a cell is dependent on its...construction and the cell it’s infecting, getting these particles to infect cells is also very titer-dependent... particle to infect a cell, it has to come into physical contact with that cell. Since there is a limited...of surface area on each cell, the way to get more AAV in contact with the cell is to increase the AAV ...between the cell and any individual AAV particle is rare. But simply getting into the cell may not always...particles that have entered the cell now have to travel up the axon, through the cell, and potentially spread ...

...this approach is limited to protoplast cells (plant cells whose cell wall has been removed). Another common...expression is dependent upon the cell type in question. In plant cells, gRNA expression is achieved by ...exposed to plant cells. The region of the Ti plasmid between the T-DNA borders (yellow) are recognized ... shown to be excellent alternative genome editing technologies especially in human cells. In contrast ... 2019) or cell-penetrating peptides (Santana et al., 2020) and plants viruses (barley yellow striate mosaic...are necessary to enable genome editing in plant cells.   This blog post will present a general overview...are necessary to use the CRISPR system in plant cells. Like other model systems, expression of S. pyogenes...

...). These cell-permeable Janelia Fluor (JF) dyes have excellent properties inside living cells, especially... fluorescent proteins. In-cell labeling strategies The majority of in-cell labeling strategies have two...and oligonucleotide labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores..."Imaging live-cell dynamics and structure at the single-molecule level." Molecular cell 58.4 (2015): 644...sparked a renaissance in biological imaging. Suddenly, cell biologists were no longer beholden to chemists and.... Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing fluorophore...question: In this age of GFP and its ilk, why should cell biologists talk to chemists? One reason is something...