We narrowed to 119 results for: Endog

1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ...Giovanni Pezzulo, and Joshua M. Finkelstein. "Endogenous bioelectric signaling networks: exploiting voltage...

2. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ... prevent aberrant binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your...

3. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...protocols that use antibody fragments to block the endogenous immunoglobulins and reduce the background staining...

4. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin...

5. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...biomolecules that are not proteins) or would perturb the endogenous state that you want to measure (overexpressing...

6. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had...

7. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...expressed in the neuron, the FingRs bind to the endogenous synaptic proteins allowing them to localize to...

8. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...mutations and truncations in the absence of the endogenous gene. Spatio-temporal expression of optogenetic...

9. Hot Plasmids: Summer 2025

   Type

   Blog Post

   Published

   Aug. 28, 2025, 1:15 p.m.

   ...) for easy visualization. A cleavage site for endogenous BACE1 protease allows VAMP2-At to remain on the...

10. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...impossible if you wanted to distinguish between endogenous and exogenous versions of the same target. In...

11. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...CAPTURE CAPTURE 2.0 Biotinylation enzyme BirA Endogenous biotin ligases Expression system Stable cell...

12. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...Western blotting and PCR, depending on the level of endogenous expression. We highly recommend the use of an...

13. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells Check Out the Michael...

14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ... level observed will be dependent on both the endogenous promoter and the 3’ UTR of the locus of interest...

15. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    Published

    Dec. 22, 2022, 2:15 p.m.

    ...from a template molecule, which is usually an endogenously available sister chromatid, though exogenous...

16. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...then tested the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency...

17. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National...

18. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...new effector (Abudayyeh et al., 2016). In the endogenous CRISPR/Cas9 system, Cas9 uses both a tracrRNA...

19. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like activity (Hu et al., 1990). We commonly...

20. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ... this using Cre-Lox mouse models in which the endogenous MECP2 gene was disrupted and silenced by a Lox-Stop...