We narrowed to 237 results for: FAS
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TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....learn faster and retain more information plus your mentor will get you up to speed easier and faster when...
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Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....making experimental workflows considerably easier, faster, and more efficient than previous methods. Still...Sanger sequencing-based software tools and is also faster and easier to use. ICE is completely free for everyone... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....and safe corners in a company, less exposed to the fast dynamics and pressure which are often found in sales...role Talking to customers everyday, it can be fascinating and exciting to listen to the many individual... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....ready for purchase. But what is the easiest and fastest way to use the system in mice? We'll have a post... vitro first. In vitro screening is a simple and fast method for validating your selected gRNA’s, and ... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m.... old-fashioned at all. With the development of new fluorescent pairs and the emergence of faster measurement... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....used to refer to something that has fallen out of fashion but is still useful and most importantly, effective...labs. However, RNA interference (RNAi) remains steadfast in the background, a handy “oldie” as you might... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m...., a method called pyrosequencing. The drive for faster, higher throughput and less expensive sequencing...both time and research progress. These will bring faster, higher throughput, and potentially more accurate... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....the two best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s (slow deactivation)...and speed, so you can design, test, and innovate faster than ever. Plus, we've already taken them for a... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m.... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow the user to save...dependency is a severe limitation, but the tool is fast enough that, if you’re engaged in heavy CRISPR design... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m....reprogramming period and the plasmid will get diluted faster when cells are actively dividing. The typical drawbacks...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies ... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m....actuator pair. Ideally, indicators should possess fast voltage sensing kinetics and have no spectral overlap...named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator...visualize the Broccoli-tagged RNA. This technique is faster and less labor intensive than northern blotting... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon...-based MAGE The Nielsen lab’s CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based...possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....generate ABE8e(TadA-8e V106W), which edits ~590-fold faster than the TadA from ABE7.10 without increasing off-target...preceded by Gs Richter et al., 2020 ABE8 590x faster adenine base editing than ABE7.10 Gaudelli et... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....small protein is over-transferring, try using the faster semi-dry transfer, which works better for small...low levels of proteins. In this case, it might be faster to run the same amount of protein in every lane... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins....optimizing protein expression and purification in a fast, efficient and affordable way. Scholz et al.., BMC... -
New Coomassie Protocol Video
TypeBlog PostPublishedOct. 31, 2023, 1:15 p.m....Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol... -
Trends in CRISPR and SynBio Technologies
TypeBlog PostPublishedFeb. 4, 2015, 3:58 p.m....data on plasmid deposits and distirbution for these fast moving fields. Addgene is a global nonprofit plasmid... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog PostPublishedFeb. 6, 2025, 2:15 p.m....genes, plasmids ship out approximately three weeks faster, as no license, or license fee, will be required... -
Bringing Renewable Energy to Addgene
TypeBlog PostPublishedAug. 27, 2019, 1:08 p.m.... cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can ...