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We narrowed to 618 results for: LIS;

Showing: 81 - 100 of 618 results

  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...PubMed Central PMCID: PMC1462937. Dietzschold, B., Li, J., Faber, M., and Schnell, M. (2008). Concepts ...Sorensen, S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K....advantage of AAV vectors is that there are well-established protocols for their production and purification...AAV1, -2, -5, -7, -8 (Taymans et al., 2007), AAV1 (Hollis et al., 2008) AAV6 (Towne et al., 2010), AAV8, ...PMID: 19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R...

  2. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo interrogation... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...

  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, ...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...

  6. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...academic research environment. The “how many?” question lies at the base of all these questions as there is not... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. Having this...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they... PIs and all gave advice on how to succeed in publishing in the current academic environment. Some great...writing skills will help you in your academic publishing goals and make you a better communicator overall...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion...

  7. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making...

  8. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...PMID: 25514926. PubMed Central PMCID: PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last...difficulties with kinase specificity in vitro. The establishment of a system in which purified, recombinant protein...interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You can follow...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...infected cells, an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

  12. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa...unless you share the results with others. If you publish data generated using your antibody, it’s best scientific...

  13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ... . https://doi.org/10.1038/mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard...Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    .... Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...team.     Mathew Goodwin is a Technical Sales Specialist at Transposagen. As a gene editing enthusiast...Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent Stem Cells:... Perry, V. V. A. Steffey, K. Miller, and D. W. Allison, “Decoding the Epigenetic Heterogeneity of Human...

  15. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...

  16. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...22205518. PubMed Central PMCID: PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with dual binary transcription...multiple genes at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not ...doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a ...

  18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ... N., Ma, W., Liu, X., Cai, Y., Kou, J., Wang, J., Li, L., Peng, S., Xu, Z., Zhang, W., Qiu, Y., Wu, Y....the first paper identifying a binding receptor published this year (Dhungel et al., 2025)! It can transduce...contributing their expertise! This post was originally published in January 2025 and updated in August 2025. References...

  20. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...plasmids from this paper at Addgene. 6. Xing, Hui-Li, et al. “A CRISPR/Cas9 toolkit for multiplex genome...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...
Showing: 81 - 100 of 618 results