We narrowed to 109 results for: LYC
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TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution to each...
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Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....downstream.4 The "cleavage" occurs between the Glycine and Proline residues found on the C-terminus meaning... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts and ... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once: Dip your... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....standard for reference and to add a loading dye with glycerol to your samples before pipetting them onto the... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....generation of the transfer vector. Q5: What envelope glycoprotein will be expressed on virus particles? A5: This... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin screen... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture ... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m.... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m.... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated ... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....unstructured peptide and therefore consisted of glycines, serines and other small, non-aliphatic amino ... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....post-translational modifications on those proteins, like glycosylation patterns. Surface labeling is particularly useful... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....are directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m.... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m.... usually 15-20 amino acids long and made up of glycine and serine with dispersed hydrophilic residues ... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....transiently using a standard detergent, Polyethylene Glycol (PEG), although the application of this approach... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....ataxia telangiectasia, congenital disorder of glycosylation, and Niemann-Pick disease type C. Although the... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m.... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG If you need more...