We narrowed to 170 results for: Library

...both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses... O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11...

...genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a...

...human miRNAs. Weiss’s collection consists of a library of reporter plasmids, each containing two fluorescent...used for high throughput screens using pooled libraries. Find the CHIME plasmids at Addgene. LaFleur et...

...CRISPR - from nickases to base editing to pooled library screening. It also has information for planning...

...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale...that can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been developed...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering... plasmids, or choose from a growing number of libraries containing pre-constructed standardized modules...

...variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with a relevant...certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, but these...

...pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode...

...so that they are easily distinguished. The UCLA Library offers some Powerpoint based templates with different...difficult to read. The Thompson Rivers University Library provides useful tips on how to craft this type ...

...Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular Biology...

...DNA-based materials, including our popular kits and libraries. This DNA-based materials repository contains ...

...paper uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest...

...-Cas9 genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823...

...multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification...

... Data repository From the Frederick National Library for Cancer Research Human Luekocyte Differentiation...

...increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple...

...prime editing efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict...high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled to their target allowing...

...PHP.B was created by first generating a diverse library of AAV9 mutants that had a small randomized peptide... loop inserted in the AAV9 capsid. This mutant library was then screened in vivo in C57BL/6J mice strains...

...to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual genes... activator were transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks...

... coming soon! We’ll also be building our video library all summer long, with additions to our other video...

...for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening ...