We narrowed to 169 results for: Library
-
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....looking ahead, we’re starting to build saturating libraries of all possible mutations in many major cancer...our Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About Cancer Immunotherapies...
-
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses... O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11... -
It’s CRISPR Week at Addgene!
TypeBlog PostPublishedMay 13, 2019, 12:01 p.m....CRISPR - from nickases to base editing to pooled library screening. It also has information for planning... -
22 Hot Plasmid Technologies from 2014
TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m....Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale...that can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have been developed...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering... plasmids, or choose from a growing number of libraries containing pre-constructed standardized modules... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with a relevant...certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, but these... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available. These libraries encode... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....so that they are easily distinguished. The UCLA Library offers some Powerpoint based templates with different...difficult to read. The Thompson Rivers University Library provides useful tips on how to craft this type ... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....DNA-based materials, including our popular kits and libraries. This DNA-based materials repository contains ... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m....Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular Biology... -
8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....paper uncovering ancestral bias in CRISPR guide library design. dCas9-directed DNA Demethylation: Guest... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....-Cas9 genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823... -
Antibody Data Repositories and Search Engines
TypeBlog PostPublishedOct. 31, 2024, 1:15 p.m.... Data repository From the Frederick National Library for Cancer Research Human Luekocyte Differentiation... -
CRISPR Cheat Sheet
TypeBlog PostPublishedJan. 27, 2021, 2 p.m....increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target multiple... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....prime editing efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict...high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled to their target allowing... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....PHP.B was created by first generating a diverse library of AAV9 mutants that had a small randomized peptide... loop inserted in the AAV9 capsid. This mutant library was then screened in vivo in C57BL/6J mice strains... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....to implement in genome-wide screens over cDNA libraries. However, using CRISPRa to target individual genes... activator were transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks... -
Summer Fun at Addgene!
TypeBlog PostPublishedMay 12, 2022, 1:15 p.m.... coming soon! We’ll also be building our video library all summer long, with additions to our other video... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog PostPublishedNov. 12, 2014, 3:54 p.m....for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening ... -
Make a Splash: Notions of Scientific Impact Are Evolving
TypeBlog PostPublishedDec. 5, 2014, 2:54 p.m....allowing researchers to track and share their own libraries and offering another way to consider and quantify...