...targets with different colors. Multiplex immunofluorescence Speaking of different colors, having a palette ...secondary antibodies in a few different species and colors makes it easy to perform multiplexed IF. By using...

...limited selection of fluorophores. If the available colors are already reserved for other markers in your ... all cells positively labeled with the selected color will not be CD8+ T cells and we can simply “dump...undesired cell populations. Dump gating uses the same color for different markers, making it easy to gate on...

...with many challenges, researchers have begun to explore genome editing approaches for exon skipping. Addgene...diseases will require HDR-mediated precision editing tailored to smaller patient populations. To bring DMD CRISPR... Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target editing. Short-term CRISPR...

...rural Africa Once this position ended, I wanted to explore another area which I was really passionate about...returning from Ghana at the beginning of 2010, I explored options for going into parasitology but another... for young scientists and PhD students to also explore a range of alternative careers outside of acidemia...

...cytometry experiment uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers...versatile and some can accommodate up to 30 different colors. This lends flow cytometry particularly well to...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...

...is a PhD trained scientist at the University of Colorado, Anschutz Medical Campus.  He studies viruses ...currently a postdoctoral fellow at the University of Colorado Anschutz Medical Campus in Aurora, CO where she.... Erin is also the President of Project Bridge Colorado, an advocacy initiative that seeks to bridge the...

...an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps, activation...activation of which results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action...

...Cas9 in the apo state (PDB ID 4CMP) with domains colored as in Figure 1. HNH, red; RuvC, orange; CTD, yellow...mismatches between base pairs 18 and 20.   Pro tip! The Taylor lab mutated the residues in this loop to produce... L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis for mismatch surveillance...

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...]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand break (DSB) approach at...ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...

...fluorophore tagging vectors for your single or multi-color imaging experiments. Interested in epitope tags?... Julian Taylor-Parker...

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...more details on how to make the most of these exploratory conversations. If you set yourself a goal of ...to Find Funding For Your Research University of Florida: Training Grants and Other Funding Opportunities...