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We narrowed to 447 results for: PAN

Showing: 81 - 100 of 447 results

  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA...With the expanded targeting ability using both systems, the authors look forward to the expanded use of ...precise RNA editing. The addition of RESCUE further expands the RNA editing toolkit and sequences that can ...

  2. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... ideas heard.  So how can we assure that all participants feel comfortable sharing their ideas? And how...unique solutions to the same problem. If your lab/company/team is still working towards a culture of encouraging...just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone could fill out the...of which, when implemented, impacted the entire company. It is important to note that the person who submitted... is an incredibly innovative group. Our entire company benefits from their policy! Perfectionism When ...

  3. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...necessarily mean that you are a future employee of the company. It does, however, offer you an opportunity to ...like to work, get online and take a look at the company or institution’s career page, where you’re likely...openings. A good approach is to do this first for companies at which you’ve already made a personal connection...result in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying...development center, if one exists. Get online! Review company and institution career pages. Make yourself visible...

  4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...sequence derived from the filamentous phage F1 (Trapani et al., 2014). Drawbacks: The choice of homology...need to be optimized for each unique application (Trapani et al., 2015).  Expression of smaller fragments...Y, Lai Y, Duan D (2008) A Hybrid Vector System Expands Adeno-associated Viral Vector Packaging Capacity...19:36–45 . https://doi.org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone...194–211 . https://doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C...

  5. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...in humans have a functional homolog in the fly (Pandey and Nichols, 2011). A huge advantage of using the...differentiation of β-cells in the the developing zebrafish pancreas. They first tagged these cells using plasmids....β-cells that originate in different regions of the pancreas and produce different levels of insulin (Hesselson...Distinct populations of quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling...113.8 (2016): E1074-E1081. PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease...

  6. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...-actin in panel A. Indeed, the quantification in panel B is not especially linear. In panel C, two different...with permission from Elsevier. In this figure, panels A–B show the housekeeping protein approach. By ...measurements are linear. Note the different y-axes between panels B and C, indicating that β-actin is less sensitive...

  7. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics to treat neurodegenerative...law. He said, “you know, the patent lawyer at my company seems to really like what she does.” I thought ...patent counsel and ran a group at two fairly large companies, Genzyme and Biogen. They’re very different jobs...corporate work, and negotiating contracts with other companies. It's one of those "every day is something different...

  8. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ...Seeding Labs, a nonprofit founded in 2007, strives to expand the opportunities and scientific resources available...first step; going forward, Seeding Labs hopes to expand its activities by facilitating connections to mentorship... assistance, and more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s...regions like South America and Africa, but we need to expand our efforts to find new and creative ways to reach...

  9. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...Drs. Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain however...Ventures, Koeris helped change Novophage's focus. The company went from developing therapeutics to developing...pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6. To...personally take on the risk of founding a startup company? 32:20 - 34:37 : Whose responsibility is it to ...

  10. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...science more accessible and reliable. We continue to expand our educational content supporting the tools in...the Institute for Protein Innovation (IPI), we expanded a growing collection of thoroughly characterized...Packaged on Request, a service that dramatically expands and simplifies your viral vector options. Almost...Through our growing community on Bluesky, our expanding educational resources, and our commitment to global...

  11. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...training, I was gradually introduced to Addgene as a company and given more details on what my position entailed.... The official training process was lined out to span the first three months of my six month employment...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...they do and how it relates to other teams at the company. This provides new (and old) employees with information...

  12. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...Bottom panel, right) A highly expressing RUBY plant is a vibrant red/magenta while (bottom panel, left)...plant beginning to germinate. (Top right, second panel) Transformed seedlings can be selected without antibiotics...developing seeds from a homozygous RUBY plant. (Bottom panels) RUBY expression can vary from different transformation...

  13. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...first sent a pre-workshop questionnaire to the participants to learn about what they were looking to learn... content. In the middle of the workshop, the participants took part in a protocol drawing exercise. The...workshop also used a shared note-taking doc where participants could share their notes together. Figure...viewed publicly and stored in one place if the participants used the conference hashtag after the conference...

  14. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,... if any additional resources are needed. All-Company Meetings: As your organization grows, so will the...to address one point aloud. This keeps our all-company meetings short, informative, and pleasant. Email...timely feedback on each other’s projects. For all-company meetings, monthly still works best. Figure out ...

  15. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...emission wavelengths. Renilla luciferase from the sea pansy (Renilla reniformis) emits a blue light with a peak...gracilirostris) 455 19 Coelenterazine None Yes Luminous Japanese ostracod(Cypridina noctiluca)Sea firefly(Cypridina...62 Cypridina (Vargulin) luciferin None Yes Sea pansy(Renilla reniformis) 480 36 Coelenterazine None No...firefly(Photinus pyralis) 560 61 Luciferin ATP No Japanese firefly(Luciola cruciate/lateralis)Italian firefly...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...hardware. Upper left panel shows the path of light through a microscope. Lower left panel shows an image of...and can allow for innovative organizations and companies to provide problem-solving platforms.     ... monocyte with planes and hardware to it. Right panel shows workflow of open hardware.    Conclusion...

  17. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...name suggests, a single color from your antibody panel. They are made up of compensation beads and a conjugated...for every fluorophore you intend to use in your panel. Using single color controls On a flow cytometer... the reference plots provided by the antibody companies or check the literature. Pro tip! Early gates,...consider using an isotype control. Pro tip! Many companies will have appropriate isotype controls linked ...

  18. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...repurposed for SARS-CoV-2 RNA detection. Early in the pandemic, labs around the world have been modifying these...viral proteins In the early weeks of the COVID-19 pandemic, the Krogan lab at UCSF generated lentiviral vectors... while many were developed during the COVID-19 pandemic. Our mission to accelerate research through sharing... up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2 pseudovirus plasmids ...

  19. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman and...advocacy skills “bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org...productive routes for advocacy efforts and help participants develop the efficacy of their advocacy skills...effective advocacy strategies on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...each year. (The dip in 2020 is likely due to the pandemic; for more discussion on that, see Pyhtila et al...CRISPR plasmids. But many other countries, from Japan to Australia, are significant depositors and requestors...CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse..., S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse...
Showing: 81 - 100 of 447 results