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We narrowed to 342 results for: SEF;

Showing: 81 - 100 of 342 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...off-target activities have proven themselves incredibly useful for the efficient generation of individual (and...systems for high-throughput screening are vastly useful for mapping the combinatorial genetics that underlie...

  2. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...as clinical therapy. These biological tools are useful because they’re derived from viruses that can infect...0.5% peracetic acid, 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher...

  5. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...it easy to spread out your samples. This can be useful if you’re transferring samples from 96 wells plates...machines I have used in the lab. It’s especially useful if you want to avoid sore fingers. There are other...

  6. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...a gene of interest. These plasmids will be most useful for constructing intermolecular FRET probes, where...acceptor fluorophore on the same protein and are useful when a process affects the conformation of the ...

  8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...insert sequences or annotated Genbank files that are useful for more complicated plasmids, like those that ... assemblies. If we obtain an NGS result that is useful, but not 100% complete, we will still make this...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...easily visualized.  The tool-kit consists of highly useful vectors compatible with Gibson and Saptrap cloning... includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag...

  10. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ... easier to work with. In either case, it can be useful to run a restriction digest of your amplified library...general pDNA Library Amplification protocol are useful references. Skim through our Pooled Library page...

  11. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...community?  Addgene exists to help researchers share useful tools, and as such, we’re always looking to understand...

  12. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... for improvement. These sensors still have many useful applications, though, and if you’re in the market...to the membrane, otherwise they aren’t all that useful. The cellular localization component is typically...

  13. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...inducible promoters. Inducible promoters are extremely useful if a desired protein product is toxic to cells ... with labelled or modified amino acids that are useful in different downstream applications. Cell-free...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...picking is shown in figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning... cloning method The agarose technique should be useful to scientists who wish to rapidly isolate individual...

  15. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...cloning, sequencing, and genotyping. It’s extremely useful but is not as quantitative as other methods of ...primers all in one PCR reaction. This is an extremely useful PCR method that can help save time and effort in...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...about these two new AAV capsids and how they can be useful tools for studying the CNS and PNS! What is the...PNS (figure32, tables 1 & 2). These capsids are useful tools for neuronal tracing and morphology studies...

  19. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... Low Combines both words and images; especially useful for conveying and contextualizing data. Often overlooked...limited to a single page of information. Primarily useful for data-focused stories. In-person lectures...
Showing: 81 - 100 of 342 results