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We narrowed to 193 results for: SPL

Showing: 81 - 100 of 193 results

  1. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., ...and higher contrast than previously developed NIR split probes (Tchekanda et al., 2014, Filonov et al., ...

  2. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...to a number of rare genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital...Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. ...

  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... effective in both NHPs and mice, but they can display species-specific tropism. For example, AAV9-X1.1...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive..., lung, and thymus, and transduce the heart and spleen at similar efficacy levels to AAV9 (Stanton et ...

  4. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...tend to be choc-full of open reading frames that display little to no homology to anything else, making ...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments...biocytin-filled cell expressing BiPVe3-dTomato, displaying characteristic PV basket cell morphology. Adapted...

  6. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue or orange-blue rather ...>Replace Red with Magenta. Or you can use Color>Split Channels and then Color>Merge Channels into whatever...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic.... Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing ...

  8. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial ...Nevertheless, amiRNA-based RNAi experiments routinely display highly specific and robust gene silencing (following...

  9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...prevent off-target expression of the sensitive FLEx-split TVA receptor used for tracing neural pathways was... with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and Cell Tropism after ...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...emission maximum. Note, you can also use the tool to display excitation spectra, which I excluded here for better...created through the actual APC-Cy7 fluorophore but displays an emission spectrum that overlaps with APC-Cy7...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...because the top figure shows the construct from our 'Split RNA' design, where the pre-processing form of crRNA... more details. One important note is that this ‘split RNA’ design is not as efficient in genome editing...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...membrane and gel during the transfer, while dark splotches could indicate dirty equipment, degraded or improperly...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

  13. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...improved peroxidase reporter, APEX2, through yeast display. APEX2 has been shown to exhibit superior performance...functional complementation between two non-fluorescent split GFP fragments. When the two fragments, expressed...

  14. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...More #agarart from our lab @SILS_UvA 🧬🧫 Soon on display @Micropia! #SciArt #MerryChristmas #happyholidays2018...

  15. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...Dr. Anne Madden created a microbe advent tree displaying over 25 archaea, bacteria, fungi, and viruses...

  18. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...important advance was quickly followed by even splashier news: Jiahao Sha of Nanjing Medical University...

  19. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...

  20. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva (a.k.a. FOP), that, ...
Showing: 81 - 100 of 193 results