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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 189 results for: SPL

Showing: 81 - 100 of 189 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments...biocytin-filled cell expressing BiPVe3-dTomato, displaying characteristic PV basket cell morphology. Adapted...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...tend to be choc-full of open reading frames that display little to no homology to anything else, making ...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...

  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic.... Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing ...

  4. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial ...Nevertheless, amiRNA-based RNAi experiments routinely display highly specific and robust gene silencing (following...

  5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...prevent off-target expression of the sensitive FLEx-split TVA receptor used for tracing neural pathways was... with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and Cell Tropism after ...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...emission maximum. Note, you can also use the tool to display excitation spectra, which I excluded here for better...created through the actual APC-Cy7 fluorophore but displays an emission spectrum that overlaps with APC-Cy7...

  7. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...because the top figure shows the construct from our 'Split RNA' design, where the pre-processing form of crRNA... more details. One important note is that this ‘split RNA’ design is not as efficient in genome editing...

  8. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...membrane and gel during the transfer, while dark splotches could indicate dirty equipment, degraded or improperly...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...improved peroxidase reporter, APEX2, through yeast display. APEX2 has been shown to exhibit superior performance...functional complementation between two non-fluorescent split GFP fragments. When the two fragments, expressed...

  10. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...Dr. Anne Madden created a microbe advent tree displaying over 25 archaea, bacteria, fungi, and viruses...

  11. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...More #agarart from our lab @SILS_UvA 🧬🧫 Soon on display @Micropia! #SciArt #MerryChristmas #happyholidays2018...

  14. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...important advance was quickly followed by even splashier news: Jiahao Sha of Nanjing Medical University...

  15. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...

  16. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva (a.k.a. FOP), that, ...

  19. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...from other commonly used FPs, and GFP does not display auto-photoconversion as do some FPs. "While this...

  20. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...Biology, I would stop by later this year when it is displayed!).  The Addgene themed music playing all night...
Showing: 81 - 100 of 189 results