Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 351 results for: SUA

Showing: 81 - 100 of 351 results

  1. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...use an RNA guide to target other nucleic acids—usually DNA—to cut, nick, or bind. DNA CRISPR array: The...previously encountered foreign nucleic acids. The unusual sequence of these arrays, with alternating spacers...

  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...genetically fused to fluorescent proteins and used to visualize target antigens in living cells or tissues. sdAbs...the process started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable...

  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ... called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers (collectively known...optimal Rluc8-Gα construct for two of the 16 non-visual G protein transducers. To use the TRUPATH system...

  4. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...labeling fewer cells in a population, it’s easier to visualize individual/non-overlapping cells. While transgenic...Drosophila, the SPARC systems can be used both for visualization and for optogenetics. The Clandinin lab used...

  6. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...seminar speakers. My eye was caught by a couple of unusual job openings, although they seemed too far afield... a look at our news editors. A fondness for visualization of science? Look at our graphics and design ...

  7. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...delivery to be viral-mediated. Transformations are usually bacterial in nature. All three of these methods...packaging plasmids into a viral production cell line, usually HEK-293 cells. When all of these viral plasmids...

  8. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...working with antibodies.) Conformation changes usually fall into one of two categories: ones induced by...or fold in certain conditions? It can help to visualize an abstract protein during your sample prep process...

  10. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...second stain, safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram...classification system is incredibly helpful in visualizing differentiations between bacteria, leading to...

  11. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...transformation efficiency. For a typical library, Addgene usually does four to eight electroporation reactions using... this representation is most easily understood visually. As an example, the curves from our recent amplification...

  12. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...'m exposed to many scientific communities, but usually I only get to scratch the surface of different ...takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint effort of five ...

  13. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...resume should be about. With a CV, information is usually presented chronologically. With a resume however... an asset,” mention “speaks French.” Employers usually don't spend more than 20-30 seconds on a resume...

  14. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ... person.  In an interview setting, this means persuading an employer that your past work has prepared ...the world to do it.  In the lab, it might mean persuasively articulating why the results you've measured...

  15. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... PAM sequence NGG TTTV PAM site preserved? Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer... differences in copy number.  And there are the usual transfection drawbacks: transient expression and...

  17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...digests to analyze plasmids, which can help you visualize this process, or visit our restriction analysis...a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers are especially...

  18. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ... methods of neuronal tracing (Fig. 1): one to visualize neurons that send information to a region (retrograde...retrograde tracer, Fig. 1A), and another to visualize neurons that receive information from a region (anterograde...

  19. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...switched R-mAbs, multiple targets could now be visualized in a single experiment, which had not been previously...especially exciting for scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex...

  20. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...they don’t require an excitation light source (usually a laser in fluorescence assays), which means luminescence...phototoxicity and even allowing multiple proteins to be visualized simultaneously. Complementation systems for examining...
Showing: 81 - 100 of 351 results