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Showing: 81 - 100 of 511 results

  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...follow our timelines! Prepare a research plan to set yourself up for success prior to your departure for...reagents are onsite before you need them, and you are set up for success. If you are communicating with a professor...

  2. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Back then, it consisted of “wooden slab benches” set on top of filing cabinets, a rickety old incubator...videos, while some are looking to develop new skill sets. Kellen Andrilenas is a Ph.D. grad student at Boston...as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located next to ...

  3. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...Colorado Boulder recently developed “Riboglow,” a set of tools that can be used to visualize RNA localization...colony screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ... don’t get a chance apply it in an interactive setting. The problem is…how can you make a “hands-on” ...clinical disease case. Then, each team received a whole set of cards (Promoters, Inserts, Tags, Origins of Replication...

  5. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ... or create custom protein collections (such as a set of FPs that you or your lab use frequently) for the...the information in the database relating to that set of proteins.  For computer-savvy users looking to...

  6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...fluorescence-based methods require a standard curve, a set of samples with a known DNA quantity and their corresponding... DNA prep. Though this method takes more time to set up in the lab, you might not have to do the calculation...

  7. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    .... Purpose Gram staining can be used in medical settings to determine the general type of bacteria present...classification system is also useful in a laboratory setting. When designing a plasmid to be inserted into a...

  8. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...information and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual...

  9. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) ... emiRFPs in your applications.  There is a whole set of spectrally distinct (e)miRFPs available. The numbers...Methods 16, 171-174 (2019). Matlashov, M.E. et al. A set of monomeric near-infrared fluorescent proteins for...

  11. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...now available in lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA...the Addgene Advisory Board) says: "When you're setting up to do a genetic screen, you need to focus on...

  12. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...get you started, here are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins...catalytically dead version of the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier...

  13. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    .... An important difference between the original setting and the new one, previously unaccounted for, may...may lead to different results. Different setting, different population, new genetic variation, or different...results of all four of those tests and not just the subset of those that return a particular result. Anything...

  15. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...communication to another person.  In an interview setting, this means persuading an employer that your past... to ask and answer these questions for a diverse set of audiences.  Every scientist needs to be able to...skills in science communication will be a positive asset throughout your career? 1. Make scicomm practice...of this as a jumpstart or a refresher for your skillset rather than a permanent fix. Treat every interaction...

  16. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...instructions use NEB buffers. Nonetheless, if you are setting up a double digest with NEB enzymes, the app is...of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence... can’t guarantee every reagent in your chemical closet is in this app. However, if you get quizzed randomly...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash...obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...the MIT Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on ...model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...now available at Addgene, so on your marks...get set...RUSH. Boncompain et al., Nat Methods 2012. Mar...; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector that enables protein...

  19. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ... based on the identity and order of a particular set of inputs, may be used to better understand and engineer...on the state cannot be undone and also limit the set of possible downstream states that can be achieved... register to record all permuted substrings of a set of chemicals. Specific examples of arrangements that...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that uniquely...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...or circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden...unpredictable cases, MoClo provides two answers: first, sets of parts with characterized behaviors are available...
Showing: 81 - 100 of 511 results