...fluorescence-based methods require a standard curve, a set of samples with a known DNA quantity and their corresponding... DNA prep. Though this method takes more time to set up in the lab, you might not have to do the calculation...

.... Purpose Gram staining can be used in medical settings to determine the general type of bacteria present...classification system is also useful in a laboratory setting. When designing a plasmid to be inserted into a...

... don’t get a chance apply it in an interactive setting. The problem is…how can you make a “hands-on” ...clinical disease case. Then, each team received a whole set of cards (Promoters, Inserts, Tags, Origins of Replication...

... or create custom protein collections (such as a set of FPs that you or your lab use frequently) for the...the information in the database relating to that set of proteins.  For computer-savvy users looking to...

...Back then, it consisted of “wooden slab benches” set on top of filing cabinets, a rickety old incubator...videos, while some are looking to develop new skill sets. Kellen Andrilenas is a Ph.D. grad student at Boston...as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located next to ...

...follow our timelines! Prepare a research plan to set yourself up for success prior to your departure for...reagents are onsite before you need them, and you are set up for success. If you are communicating with a professor...

...Colorado Boulder recently developed “Riboglow,” a set of tools that can be used to visualize RNA localization...colony screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018...

...information and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual...

...kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but...

... at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) ... emiRFPs in your applications.  There is a whole set of spectrally distinct (e)miRFPs available. The numbers...Methods 16, 171-174 (2019). Matlashov, M.E. et al. A set of monomeric near-infrared fluorescent proteins for...

...get you started, here are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins...catalytically dead version of the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier...

...communication to another person.  In an interview setting, this means persuading an employer that your past... to ask and answer these questions for a diverse set of audiences.  Every scientist needs to be able to...skills in science communication will be a positive asset throughout your career? 1. Make scicomm practice...of this as a jumpstart or a refresher for your skillset rather than a permanent fix. Treat every interaction...

... all of biology) require large data sets. Whether these data sets include videos of behavior, many many...

.... An important difference between the original setting and the new one, previously unaccounted for, may...may lead to different results. Different setting, different population, new genetic variation, or different...results of all four of those tests and not just the subset of those that return a particular result. Anything...

...now available in lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA...the Addgene Advisory Board) says: "When you're setting up to do a genetic screen, you need to focus on...

...instructions use NEB buffers. Nonetheless, if you are setting up a double digest with NEB enzymes, the app is...of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence... can’t guarantee every reagent in your chemical closet is in this app. However, if you get quizzed randomly...

...DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, ...

...fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash...obtained through Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible... Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche et al., Nat ...the MIT Synthetic Biology Center have deposited a set of 11 phage integrases which act irreversibly on ...model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...now available at Addgene, so on your marks...get set...RUSH. Boncompain et al., Nat Methods 2012. Mar...; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector that enables protein...

... based on the identity and order of a particular set of inputs, may be used to better understand and engineer...on the state cannot be undone and also limit the set of possible downstream states that can be achieved... register to record all permuted substrings of a set of chemicals. Specific examples of arrangements that...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that uniquely...

... many of the same elements as a STAR answer: a setting, a conflict, and a resolution. Stories usually ...interviewers will not have the same experiences as you, so set them up for success by giving them sufficient background...