... in the space and decided to reactivate it. We started out by holding a few journal club events and soon...equipment and safety training prior to getting started. For example, Timothy Stiles joined BosLab last...Community projects at BosLab Not all of our members start out with personal projects in mind. Thus, to encourage...Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering Project (OYEP). Through...

...with the people in your life. At Addgene, we’ve started virtual activities like team lunches, a virtual...your work online. Maybe you’ve always wanted to start using social media professionally but haven’t had...even if your defense isn’t for a while. Getting started early on introductions and literature reviews could...best practices in your research. For a place to start, check out Reproducibility for Everyone. Plan out...

...about Synthetic Biology. Can you tell me where to start?” is a question more and more students are asking... principles to biological systems. Today, what started in Cambridge with a handful of local high school...synthetic biology club of its kind, running from start to finish during the academic year and remaining...Science Department, or to simply tour the lab as a starting point into further STEM exploration.  Further ...

...grateful, I started setting aside 3-5 minutes to sit quietly at my bench before the start of any experiment...experiment. What fixed it was not trying even harder to start earlier, but simply moving all my prep work to the...resources on Addgene’s blog A Guide to Getting Started in Undergrad Research Early Career Researcher Toolbox...

...same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA Perhaps you think...scientific terms when socializing at a conference, but start with a more general intro until you know if the ...problems.  During the conference The conference may start in the airport. If you see someone traveling with...and put your phone away (did I say that already). Start with, “Is this seat taken? Can I join you?” Next...

... drug resistance Although Bhang and co-workers started using the tool to look at a variety of questions...preexisting in the parent population prior to the start of treatment. They hypothesized that, if they labeled... a researcher to label individual members of a starting population of cells with specifc, easily read ... to both drugs. The future of ClonTracer Since starting her new role as an investigator in the In Vivo...

...robot and to find out how you can get one. As a starting point, I would recommend conferences like Lab ... than you ever knew existed! Addgene recently started automating a few protocols to make standard lab...about 200 vials in 45 minutes by hand. How can you start automating your lab? You might not be doing enough...can do to get into the robotics spirit include: Start using electronic multichannel and single channel...

... locus, scientists can mutate the transcription start site, splice sites, exons, and/or promoters to knock... of exons. Disrupting promoter or transcription start site function would also help to reveal the impacts...polyadenylation signal downstream of the transcription start site will terminate transcription of most of the...based approaches to study ncRNAs. (A) Transcription start site (TSS) deletion. (B) Mutations of splice sites...

...antigenic drift.    Starr et al Cell. 2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science....RBD DMS Library generation and sequencing. From Starr et al Cell., 2020. (B) Mapping antibody-escape mutations... mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV-2 infects host...

... on the hood for at least 15 minutes before you start any tissue culture work and make sure that clean...as well as that watch you’re wearing before you start. Also, avoid unnecessary talking because talking... the hood and can wipe them all down before you start. This will also help you avoid moving things in ...split very soon. There is power in numbers! When starting off with a new cell line, grow the cells, aliquot...

...plan and optimize your own protocols. Here we’ll start with just the first couple of steps - mainly, fixing... a protocol is available. When possible, try to start with conditions and reagents that have already been...for general IF protocols for your sample type. Starting with a protocol that usually works in your system...minimize the risk of it being lost from your sample, start by fixing with formaldehyde and permeabilizing with...

..., each flanked by a transcription start signal sequence (gene start) and a transcription termination/polyadenylation...luciferase or eGFP) flanked by the EBOV-specific gene start and gene end sequences. Thus, the minigenome contains...luciferase). The polymerase recognizes the gene start signal and initiates transcription of the reporter...

...production steps before you can start transducing your virus. Let’s start there!     Figure 1: ...services that can prepare the libraries for you.  Starting from plasmids requires amplification, verification...disrupting plasmid representation. A good place to start is with a protocol for a library of similar size...

...reporter proteins. Protein translation requires the start codon of AUG immediately downstream of a kozak sequence...generated a silent GFP construct, that contains an ACG start codon mutation, and integrated it into human and...As the initiation of protein translation at the start codon ATG is universal, the Dow lab successfully...vivo. As GO relies on the correction of a mutated start codon to initiate protein expression, it can be ...

...forget to start it, the samples can actually migrate into the buffer — so it’s better to start over if ... runs to optimize or troubleshoot.   Before you start… Mark your membrane! Membranes look the same from...optimize your antibody dilution further from this starting point. You can also consider using polyclonal ...than blots used to confirm overexpression. Before starting a western blot, think about how you’ll interpret...

... longer than necessary before starting your experiment, or if you start an experiment before adequate ... or frt-STOP-frt (FSF) sequence prior to an ATG start codon to prevent transcription in cells where the...the serotype, except now you will already have a starting “optimal” injection volume and titer to begin ...

... uses of MoClo and the kits Addgene offers to kickstart these applications, no matter what your model ...isolation, then the MoClo Pichia Kit will get you started (Obst et al., 2017)! It is specifically curated...reporters (Engler et al., 2014). This kit is a great starter for your general plant cloning experiments. Addgene...with a large library of common parts to get you started quickly (Fonseca et al., 2019). Recently, the toolkit...

... need to create an account with Addgene before starting the process. After you have logged in to your ...Online Deposit" or "Download Deposit Spreadsheet". Start your plasmid deposit There are two options to begin...manuscript is published in a peer-reviewed journal. To start this process, simply enter your preprint title or... Learn the Benefits of Depositing with Addgene Start Your Deposit Browse Plasmids ...

...our new AAV Packaged on Request webpage to get started with your browsing and requesting. If you’re curious...easier to go from selection to viral vector. To start the AAV packaged on request process, simply navigate...placed, we’ll facilitate the necessary MTA, then start production and quality control.  Our packaged on...

... amazing students at Washington University who started the BALSA group, a nonprofit that provides short-term...stick to timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech...by Peggy Klaus Looking for a mentor or want to start a peer mentoring group? "What Makes a Good Mentor...