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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 102 results for: THY-2

Showing: 81 - 100 of 102 results

  1. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547 Kunda, Z. (...were much more likely to rate that study as untrustworthy, inaccurate, methodologically flawed, and biased...

  2. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...therapies produce lasting side effects, damage healthy cells, and often cannot eradicate metastasis or...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...JS, Legube G, Miller KM Genes Dev. 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus...gene a megabase away, or looking at the histone methylation status at a double-strand break that needs repairing...

  4. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew...

  5. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for...overproduces hydrosulfide ions yes - Grow with methylmercury. S. cerevisiae  no no Can be used for color ...

  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover...

  7. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...introduce some of its dsRNA intermediates into a healthy individual to provoke an immune response and then...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...excise the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al...and opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually...rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected with HIV-1....

  9. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...

  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the interaction between the sender cell...targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., &...

  13. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are...of many posts in our career series.  “The only healthy communication style is assertive communication....

  14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...split at 175 Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin... Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools: Split-TurboID for protein...

  16. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...are detargeted from the liver, kidney, lung, and thymus, and transduce the heart and spleen at similar ....org/10.1038/s41592-020-0799-7 Lawler, A. J., Ramamurthy, E., Brown, A. R., Shin, N., Kim, Y., Toong, ...

  17. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...efficient rate. These cuts most often occur in thymine-rich regions on the non-target strand. Occasional...

  18. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise... for the regulatory services department as our healthy homes program was moved from one department to ...

  19. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...producing lentivirus in Lenti-X 293T cells using a polyethyenimine transfection protocol. I also recommend searching...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward...or too old. Ensuring that you are working with healthy, happy cells is one of the easiest ways to improve...
Showing: 81 - 100 of 102 results