We narrowed to 546 results for: TIL;

... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz PG. A versatile platform for single- and multiple-unnatural amino...

...tips for scientists working at home, and you will still find this useful. Now that we’ve been doing this...you are more desperate or needy so you can’t wait until you imminently need a job. Start now. Informational...edit everything you’ve tried to draft, but you’ll still be practicing and getting familiar with the process...

...episome seemed appropriate, especially since the Fertility, or F-factor discovered by Ester Lederberg in ...chromosome in some cases. This terminology held until the 1960s when scientists began to study other extrachromosomal...Although discovered in the early 1950s, it took until the 1970s for plasmids to gain prominence in the...

...accordingly until we had a model that was viable.  This is not to say that we aren’t still pitching for...Although many emails resulted in no response, I was still able to get through to a number of experts, including...

...engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex ...off switch The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter... By using an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding...

...neuroengineering, and some of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering ...focus on the next round. Addgene: What tools are still missing from the optogenetics toolbox? Boyden: That's...question. We've made a lot of progress. I would say we still would like to find ways to make these tools more...

...learning about biology in high school, it wasn’t until I started working in my first molecular biology ... like taking an engine out of the car – you can still use the engine as a power source, but now you don...pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production proceeds. ...

... alcohol or acetone drop by drop onto the smear until the alcohol runs clear and immediately rinse. Flood...for 30 - 60 seconds. Rinse the slide in tap water until the runoff is clear, blot gently to dry, and then...by the interaction of CV+ and I- in the thick multilayered membrane of the gram positive bacteria. In a...

...limitation. The procedure will also need to be perfected until the efficiency of repair approaches 100 percent.... fundamental questions about CRISPR/Cas biology still left to answer as well, she says, and those details...increasing effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of ...

...played enormous roles in enabling researchers to utilize and develop these tools by making their own fluorescent...loops on either side of an enzyme’s active site and still block enzyme function upon exposure to ultraviolet...Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety of research applications...

... That papers with plasmids deposited at Addgene still accrue on average 2 to 3 times as many citations...step. There are lots of other factors that could still be controlled for. As an example, plasmid-related...of correlation. But even with that caveat, it is still suggestive that we see such a big difference in ...

...community-driven life of its own. Nevertheless, there are still many open questions to be clarified, especially ... put their work on the shoulders of giants. The still relatively young movement of sharing open hardware...Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard...

...requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because of its lowered editing...coexpressed proteins run as ladders. Image from Santilli, et al. 2021 Plasmids are used to express ...via engineered immunoglobulin binding domains.  Santilli, R.T., et al. Sci Rep (2021). https://doi.org/...

...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression strategies...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ...

..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...genetic approaches. In addition, the dTAG system is versatile and can be used in both cell-based assays and ...

...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...time point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli...

...however, have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure ...located in the midbrain, fiber-optic cannulas were still stereotaxically implanted into the mice’s brains.... Excitation of neurons deeper in the brain may still require fiber-optic cannula implantation, but altering...

...effects. Luckily for us, CRISPR is an incredibly versatile system that can be tweaked to target ncRNA. CRISPR...and its role in gene expression, though this may still unexpectedly affect nearby genes (Figure 1A-C). ...lncRNAs. The Cas14 variant is a smaller and more versatile option. It can be used to target lncRNAs with ...

...done significant work to improve the Adv genome’s utility in research and its therapeutic potential. Note...use of this system. New and improved systems are still being developed. A detailed protocol for production...should be used as an internal control (ATCC#VR-1516) until the assay is validated. Infectious titer: (Plaque...

...identifying a suitable route of administration. However, until recently safe delivery of nucleic acid cargo (DNA... of blindness and was thought to be untreatable until 2008. Following a single dose of subretinal administration... and other ongoing clinical trials support the utility of AAV as a broadly applicable gene delivery tool...