We narrowed to 136 results for: TOX

1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...

2. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...

3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...desired product while avoiding the accumulation of toxic intermediates or the shuttling of the intermediates...

4. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...labeling approach, is an alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein...

5. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...substitutions are slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in ...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...Slaymaker et al. showed that these mutants are not toxic to HEK cells and that similar mutations can improve...

7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...

8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...stop it from being used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow ...

9. Rabies and Neuronal Tracing

   Type

   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ...First, these high levels of viral replication are toxic. Second, infecting subsequently higher and higher...

10. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ... efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells...

11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... the plasmid will produce anything dangerous or toxic to humans in bacteria, select “yes” in the drop-...

12. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a ...

13. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ... long-term transgene expression and negligible toxicity. However, their production is more complex and...

14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

15. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...performed 12–18 hours post-transfection to help remove toxic transfection reagents and keep the cells happy. ...

16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ... for a single insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from...

17. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split very soon. There...

18. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016...

19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 mRNA and...

20. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...