We narrowed to 348 results for: abo.4

...Cell Stem Cell 4, no. 6 (2009): 472-76. PubMed PMID:19481515. PubMed Central PMC: 2705327  4. Hockemeyer,...reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. PubMed PMID: 21339765. Methods figures...PubMed PMID: 20139967. PubMed Central PMCID: 2892897. 4. Stadtfeld, M., and K. Hochedlinger. "Induced pluripotency...methods do you have experience using? Let us know about your iPSCs experiences in the comments below! References...Additional Resources on the Addgene Blog Learn about Methods to Reprogram Peripheral Blood Cells Check...

...expectations. (Don't think you can finish that book in 4 weeks!)” “Sometimes a mentor is a sounding board--...Connie Cepko to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this... answers to the top questions scientists ask me about mentoring. Here they are: What makes a good Mentor... Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do I ask someone...a good science mentor? I started a conversation about many of these topics with the researchers who joined...often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela Depace's...questions in future posts. If you have a question about mentoring that you want us to cover, let us know...

...futures both inside and outside of academic research (4). There are currently 17 sites around the USA undertaking...PMID: 22567149. Pubmed Central PMCID: PMC3342243. 4. Mathur, Ambika, et al. "Transforming training to ... the Jackson Laboratory for Genomic Medicine and a Senior Research Associate at the Labor and Worklife...increasing data collection about the scientific workforce, in particular about postdocs. Among other involvements...Research Symposium Boston 2015 – Academic Data and the Labor Market. For more information on the symposium as...generating too many trainees per PI” “Lack of awareness about how the system operates and functions” “Complete...available in academia (5, 6). Academic data and the labor market for PhDs Later this week, postdocs and students...

...carbohydrates added to antibodies post-translationally. 4) Modifications made to antibodies, such as inclusion...Heterogeneous Populations to Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390/antib4030197  Hegazy...Check out our post on Biotinylation to learn more about biotin and its use in molecular biology. Other Conjugates...perfectly blissful, productive lives without thinking about these chemical reactions. After all, there is an...ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

...Alternatively, glassware can be baked at 180°C for at least 4 hours.   If your final RNA sample(s) are resuspended...time it took to isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still...more foolproof (still without a kit!) As mentioned above, working with RNA requires keeping your samples ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...supernatant is extracted with chloroform as in the above no-kit method.  Researchers have also developed ...

...DNA, then they could diminish off-target cutting (4). However, instead of weakening interaction sbetween...PMID: 25079318. PubMed Central PMCID: PMC4176945. 4. Kleinstiver, Benjamin P., et al. "High-fidelity CRISPR–Cas9...With this new found structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis...edits in mammalian cells and may decrease worries about off target effects in applied and/or therapeutic...at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1 Read How Non-Homologous End Joining...

...homozygous alleles at both of your genes of interest. About 1 in 4 mice will be homozygous for the mutation at ...mice, as well as progeny from future generations. 4. Continue breeding your colony You’ll want to generate...Find our Plasmids 101 blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts... posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide Learn more...

...killer gene”. Gene. 1994 Oct 11;148(1):71-4. PubMed PMID: 7926841. 4. Bernard, P., et al. “The F plasmid CcdB...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

...Encouraging students to do science anywhere.  Chapter 4 - JEI's mission and how they assess whether or not...working in a lab for the first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing...resources Use our Plasmids 101 eBook to teach students about basic plasmid biology Use or CRISPR 101 eBook to...

...Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for ... and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central PMCID: PMC4666719...Additional Resources on the Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the...

...commercial vendors. These libraries usually contain 4-12 gRNAs/gene, which results in libraries containing...research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali...PMID: 25075903. PubMed Central PMCID: PMC4486245. 4. Ma, Hongming, et al. "A CRISPR-based screen identifies... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...

...that should work well. Quickly after transfection (4-8h), cells start releasing viral particles into the...after transfection. This supernatant can be stored at 4 °C or can be directly added to your target cells. ...Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech. 4. Walter, W., and U. Stein. "Viral vectors for gene...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...even your own! You should therefore be cautious about inserting potentially harmful genes into a transfer...

... We have been using yeast models (3, 4) to answer questions about the evolution of the genes involved ...PMID: 24807111. PubMed Central PMCID: PMC4012490. 4. Hoffman, C.S., Wood, V., & Fantes, P.A. An ancient...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques...species, but these studies generally leave questions about gene function wide open. What if two seemingly similar...question, the plasmids are also used to teach students about restriction mapping and western blotting. Students...communicating science to the public. You can find out more about Clare at the Biology Dept. site.  References 1. ...PMC4596657. 5. Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae...

...systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate...system a promoter controls the expression of a GAL-4 driver that can bind to activation sequences upstream...resources on the Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids...always emphasized the need for open science and collaboration. The field already has comprehensive reference...planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check out their...elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative polyadenylation...addition to the wealth of open resources described above, there were many plasmid tools presented at the ...

...are often looking for outreach volunteers as well. 4) Blog - Hone your writing skills Take the DIY approach... graduate course, UWE Bristol offers an intensive 4-day Masterclass in science communication. The National...Sainsbury Laboratory. She is passionate about science communications and loves talking about science, ...Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early career researcher...break into science communication? Everybody goes about this in their own way. The career paths into science...Society gives training in media skills, writing about your research and residential communication. If ...

...optogenetic implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...PMID: 23042500; PubMed Central PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics...distance so ideal implant placement should be about 0.4-0.5mm above the site of your stimulation. The scatter...scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that the majority...instead). Laser stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in...These lasers are relatively cheap and will run you about $2-3,000/laser. However, an important caveat is ...output of the laser must be controlled. As discussed above, each opsin variant has slightly different activation...

...Biology research and resources in Singapore. Chapter 4: Life as a new primary investigator. Coming up with...getting a lab up and running, and learn a little bit about what it was like for Wei Leong to work in George...

...to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too many panelists...moderator and organizers should develop a list of 4-6 pre-event interview questions. The moderator (possibly... are nice if you can afford them). I am talking about making sure the audience is interested, educated...of the stories or topics you know they can speak about. There should be some spontaneity, but having a ...location and parking information and remind them about the event as it approaches. Room set-up and logistics... make or break the discussion section. And what about those audience members that want to take over? It...

...sites interspaced with random sequences.   Strategy #4 (ProD): Create promoters with the help of epigenetic...different promoter designs, promoters from strategy #4 had the highest rate of targeted expression. Surprisingly...Addgene blog Read more about using AAV for retinal gene therapy Find more about precise neuronal targeting...injected into macaques and postmortem human retinas. About 25% of these AAV synthetic promoters successfully...

...process since May 2020. We find this process takes 4-6 weeks on average. Therefore, we recommend placing...expedite the application process.  More information about export control licenses is available through the...Industry and Security. If you have any questions about this or other Addgene shipping and ordering processes...customer support team at [email protected].  Curious about how rabies plasmids are used in the lab? Check out...