Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 231 results for: bon

Showing: 81 - 100 of 231 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  3. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...

  4. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro...Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (RNPs) Read Tips for a 1st Time CRISPR User...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element, leading to separation...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

  6. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories...-White Screening Choosing Your Perfect Plasmid Backbone  ...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...plasmid backbone that was designed specifically for transient expression (find some useful backbones here...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no genetic material, ...particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene,...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

  12. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...plays some role, and mismatches with more hydrogen bonds are more readily ligated than those with few. For...many ligases, G:T mismatches, with two hydrogen bonds and a base-pair size nearly indistinguishable from...

  13. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates...//doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient genome editing in Caenorhabditis...

  15. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...for the target:probe/primer bonds to be more stable than the hairpin bond.  Alternatively, chemistries...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...domain that promotes the formation of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...

  18. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ... over to our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted...

  19. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed right into the box. Which is...
Showing: 81 - 100 of 231 results