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We narrowed to 319 results for: bre

Showing: 81 - 100 of 319 results

  1. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...this system, Cas9 generates a double-stranded DNA break at the genomic loci of interest and on either side...complementary to either side of the double-stranded break in the genomic loci of interest. As the universal...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...

  3. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...Conveniently, the process doesn’t cause double-stranded breaks and can deliver large sequences and even entire...allowed certain mutated phages to outcompete their brethren, and the researchers isolated these more efficient...

  5. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the cleaved... of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning: 1. Create...

  6. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...research studies highlight the importance of taking a break and incorporating EAs into your routine.  Getting...learn new skills, while also getting a much-needed break from your lab bench.  Want further information ...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...it’s acceptable if it’s in the name of beer and bread. The following table lists some of the biological...end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR)....

  8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility...7). An independent team led by Hans Clevers at Hubrecht Institute in The Netherlands used the CRISPR/Cas9...

  9. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... during the day is a great way to take a mental break, while meditating right before bed will help you...it. Plan defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique...

  10. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...roles, including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

  12. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...important biological information, amino acid abbreviations, and structures into one convenient resource...say that I still have the single and triple abbreviations of the amino acids memorized, but the structures...

  13. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... Cleave target DNA resulting in a double-strand break (DSB) Cas9 and its variants have two endonuclease... which is E) cleaved leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the...

  14. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled receptor" into ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

  15. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...unedited site distal tothe predicted double strand break, and a NHEJ/ “drop-off” probe designed to bind directly... directly at the predicted double strand break site (Figure 2A). The single molecule resolution of digital...

  16. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

  17. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...

  18. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...upregulation is correlated with poor survival in breast cancer patients. By digging into the literature...containing RNAs could play a role in the severity of breast cancer. Infectious Disease. RaPID can also help...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... else to read and / or give yourself a bit of a break before you read over it again with fresh eyes and...your institute or a private funding body. Success breeds success and I have certainly found that the experience...

  20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...enhances endonuclease delivery for double-strand break repair." Gene therapy 17.9 (2010): 1175-1180. PubMed...
Showing: 81 - 100 of 319 results