We narrowed to 137 results for: ckb

1. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   Published

   Aug. 19, 2025, 1:15 p.m.

   ... a version that fits your setup: Plasmid Backbone Promoter pRSET OCaMP pRSET T7 pAAV-hSYN1...

2. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...different wavelengths. Addgene provides empty backbones with the luciferase gene, a variety of expression...

3. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...communication skillsClear communication is the backbone of effective management. Miscommunication often...

4. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences...

5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...

6. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...lentivirus. Also, note that due to the size of the backbone, transformations require electroporation. References...

7. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...promoters were also tested in different plasmid backbones that varied in size or carried different genes...

8. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...try to use an excess of insert compared to the backbone plasmid; a starting point could be a molar ratio...

9. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   Published

   June 27, 2019, 12:25 p.m.

   ...toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin...

10. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable...

11. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

12. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

13. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...

14. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough context...

15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage...

16. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ...

17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...(about 2.7kb total)—is inserted into a plasmid backbone for delivery to the cells. As a precautionary ...

18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ...

19. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    .... https://doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics of Reproducibility...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...