We narrowed to 217 results for: gnal

...found that adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can ...biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody...NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm...

...Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute... study also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect...transform zygotes into adults and explain how signaling pathways control this process. Additional zebrafish...

... Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with...illustrated the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed... opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather because of the...

...PinkyCaMP had better brightness, signal-to-noise ratio, photostability, and signal response upon Ca2+ binding...the MitoTRACER system. NLS = nuclear localization signal; TEVp = TEV protease. Created with BioRender.com...

...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it...single action potential-evoked axonal glutamate signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak...Waldron Glypicans are important modulators of cell signaling pathways during embryonic development and cancer...

...provides a high contrast image in which a bright signal overlays a perfectly black background. In addition...to more rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation...light-reducing pinhole. By collecting more photons, a higher signal to noise ratio can be achieved (6). Super-resolution...

...you‘ll need, including sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014...protein degradation on chimeric antigen receptor signaling (Kim et al., 2024). The EMMA Toolkit is another...et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory,...

...the associated fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of ... bind to a primary antibody thus amplifying the signal.       Intracellular protein targets, measured... FITC and that from PE. During compensation the signal arising from a specific fluorochrome is removed...

... do it (i.e. where the signals come from and what implications their signaling is likely to have). Anterograde...neuron to another at synapses resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015...

...improves protein solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds...Plasmids designed for these systems may include a signal peptide (SP) that routes the protein through the...

...sensitive than nLightR, both generating a measurable signal after electrical stimulation with a single pulse...pulse, while nLightR gave a measurable signal not at one pulse, but at the second test point, ten pulses...

...exactly what iRFPs achieved. Those proteins allowed a signal-to-background ratio in mammalian tissues 20-fold... In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher resolution...

...material, cells or tissue, to produce a strong enough signal over background noise. This is not terribly problematic...large numbers of cells or when a high degree of signal to noise is desired. It also provides an excellent...

...the riboswitch results in increased fluorescent signal. Prior to the development of these probes, fluorophore...less prone to photobleaching, and achieve better signal in vivo as compared to imaging with broccoli aptamers...

...molecular level. The brain is constantly influenced by signals it receives from the entire body, making it difficult...advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were very few...

...amplify fluorescent signals in in vivo imaging: the 10xHA tag increased the fluorescent signal to noise ratio...

...displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and translated...reporter. Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind...

...not need to be as high to achieve an observable signal in your experiment, therefore titer may not need...fluorescent proteins required to get a good fluorescent signal. It may not be necessary to concentrate this type...

... Monoclonal antibodies can yield relatively low signal in immunoassays due to only having one binding ...you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal, with ...

...locations, measure fluorescent signals from the sensor, and send a signal including its position in the...