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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 214 results for: gnal

Showing: 81 - 100 of 214 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...found that adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can ...biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody...NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm...

  2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with...illustrated the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed... opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather because of the...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it...single action potential-evoked axonal glutamate signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak...Waldron Glypicans are important modulators of cell signaling pathways during embryonic development and cancer...

  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...you‘ll need, including sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014...protein degradation on chimeric antigen receptor signaling (Kim et al., 2024). The EMMA Toolkit is another...et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory,...

  5. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...provides a high contrast image in which a bright signal overlays a perfectly black background. In addition...to more rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation...light-reducing pinhole. By collecting more photons, a higher signal to noise ratio can be achieved (6). Super-resolution...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...the associated fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of ... bind to a primary antibody thus amplifying the signal.       Intracellular protein targets, measured... FITC and that from PE. During compensation the signal arising from a specific fluorochrome is removed...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... do it (i.e. where the signals come from and what implications their signaling is likely to have). Anterograde...neuron to another at synapses resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015...

  8. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...improves protein solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds...Plasmids designed for these systems may include a signal peptide (SP) that routes the protein through the...

  9. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...sensitive than nLightR, both generating a measurable signal after electrical stimulation with a single pulse...pulse, while nLightR gave a measurable signal not at one pulse, but at the second test point, ten pulses...

  10. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...exactly what iRFPs achieved. Those proteins allowed a signal-to-background ratio in mammalian tissues 20-fold... In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher resolution...

  11. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...material, cells or tissue, to produce a strong enough signal over background noise. This is not terribly problematic...large numbers of cells or when a high degree of signal to noise is desired. It also provides an excellent...

  12. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...molecular level. The brain is constantly influenced by signals it receives from the entire body, making it difficult...advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were very few...

  15. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... Monoclonal antibodies can yield relatively low signal in immunoassays due to only having one binding ...you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal, with ...

  16. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and translated...reporter. Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind...

  17. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...not need to be as high to achieve an observable signal in your experiment, therefore titer may not need...fluorescent proteins required to get a good fluorescent signal. It may not be necessary to concentrate this type...

  19. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... set, we would expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed ...all organisms and that if you want the brightest signal, you may need to try several proteins in your system...
Showing: 81 - 100 of 214 results