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We narrowed to 326 results for: nans

Showing: 81 - 100 of 326 results
  1. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol...
  2. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...
  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...pathophysiological complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation...renewable sensor, a bacterium that can sense contaminants and emit light as a response. To create this...fluorescent protein transcription in response to river contaminants and transform these plasmids into a bacterial...
  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are used. For predominantly retrograde tracing, albumin protein labelled...PMC2600441. Foust, K.D., Nurre, E., Montgomery, C.L., Hernandez, A., Chan, C.M. and Kaspar B.K. (2009). Intravascular...2009). Comparison of transduction efficiency of recombinant AAV serotypes 1, 2, 5 and 8 in the rat nigrostriatal...motor neurons after intramuscular delivery of recombinant AAV serotype 6. Gene Ther 17, 141–146. PubMed...
  5. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...field-based software product manager at Oxford Nanopore Technologies. If the first thing you hear when...streamlined manner. Now, in my current role at Oxford Nanopore Technologies, I work with our customers in the...currently a Field-based Product Manager at Oxford Nanopore Technologies for the North America region. She...
  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...optogenetics, and chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application of AAV is...the deliverable DNA) are removed, resulting in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...
  7. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...embryos produced were not being used to establish a pregnancy, the researchers were able to examine each individual...
  8. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... CRISPR’s therapeutic potential in mice with a dominant cataract disorder caused by a single copy of a...have experienced hiccups (either clinically or financially), and others we just don’t have the time to talk...technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked to the liver...
  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...immunogenic profile. AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral... responses to adeno-associated virus and its recombinant vectors." Gene therapy 10.11 (2003): 964-976....
  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...nature, agriculture and bananas. You can follow her on Twitter @BananarootsBlog.   Additional Resources...engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow at The Sainsbury Laboratory...
  11. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...from the genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the...natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral vectors all have a genomic...
  12. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Cloin BMC, Finan K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun...alleviated by maximizing label density. In some cases, nanobodies have been found to help improve label density...
  13. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... genes required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball...hMDH1). They then expressed the original and recombinant enzymes in bacteria, purified them to near homogeneity...complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119...
  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under conditions commonly...Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct...Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent...
  15. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic gRNA, is incubated...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4...
  16. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...to aid in the expression and purification of recombinant proteins. Tags for stability and solubility ...hurdles to overcome in order to overexpress a recombinant protein? It is not generally in a cell’s best...glutathione S-transferase (GST), which can be fused to recombinant proteins for one-step purification with glutathione...
  17. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...results in the production of air-filled protein nanostructures called gas vesicles, which scatter sound waves... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...Maresca, D. et al. Nonlinear ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, ...
  18. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...non-specific binding or false positives.  If testing a recombinant protein, especially one with tags, be sure to...the endogenous protein, since folding of the recombinant protein may differ from the native form, which...addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...
  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ..., Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose or magnetic...their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed, which can...on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...
Showing: 81 - 100 of 326 results