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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 264 results for: nif

Showing: 81 - 100 of 264 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts to do so have...improved version of BioID, called BioID2, is significantly smaller, allows for more selective targeting...metabolically cooperating (SeMeCo) communities. The uniform multiple cloning site in the plasmid series also...

  2. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...delivering other treatments, their efficacy can be significantly increased through structural and functional ...Angelo Nicolaci is a PhD candidate in the lab of Jennifer Binning at Moffitt Cancer Center in Tampa, Florida...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... Organizing a thoughtful engaging talk takes significant effort. It requires evaluation of all of the ...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...repair of a temperature-sensitive pha-1 mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes...recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering...

  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... amplicon sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer ...networks in biological systems. NGS will also play significantly into the realm of clinical therapeutic development...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo kits are designed...j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific...

  7. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...the immune mechanisms of the host. The cause, manifestation, diagnosis, recurrence, and treatment of cancer...differ in practically every facet starting from manifestation to their specific interactions with different...

  8. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...enough.  Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist by training...supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral ...

  10. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...C-terminal end of PEmax to produce PE7. PE7 significantly improves editing efficiencies compared to PEmax...Liu's lab! This post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. ...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your antibody. ..., PhD, MRCP, from Only Good Antibodies, with significant input from Michael Biddle, PhD, also from Only...

  13. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...However, they have high cross-reactivity, vary significantly from lot-to-lot, and have to be re-validated...option to cast your own gel. Handcast gels are significantly cheaper and generate less waste. However, they...

  19. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...
Showing: 81 - 100 of 264 results