...well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples from both groups... molecular weight shifts indicative of tau phosphorylation. By including the positive control, you will...controls are single stained samples for each fluorophore and are critical to analyze multiplex flow cytometry...

... the phosphatase you choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are ...overhangs after digestion, you will need to use a phosphatase to prevent re-circularization of the backbone... treat your digested backbone plasmid with a phosphatase prior to the ligation step or prior to the gel...

...CRISPR variant going forward.   Special Delivery Fluorophore Targeting for FRET Studies While I love all of...This post describes how to target and use FRET flurophores for a variety of applications. While editing ... to study biological phenomena and about how fluorophores other than fluorescent proteins can be incredibly...Comparison to Cas9 and NgAgo Special Delivery: Fluorophore Targeting for FRET Studies Gendered Innovations...

...et al. "A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas...Deisseroth, combined optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events...rhodopsins, light-sensitive proteins that absorb photons for energy conversion or the induction of intra...resulting viral-infected neurons are subsequently photo-stimulated through fiberoptic cannulas directly ...

...started!   Thanks for all your hard work!   Fluorophores When is a Monomer not a Monomer? The Top Three...Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research...fluorescent proteins are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments...experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based machines...

...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

...assume that anyone reading this article has a smartphone nearby. We tailor these devices to our own interests...have relied more and more heavily on adapting my smartphone to help me with my research in terms of preparedness...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most...with colleagues and link your notebook from your smartphone to your computer. Lab Archives will help both...

...questions. Don't try to get all panelists on the phone at once – you won't get as much out of it and it...better at remembering these things on a relaxed phone call than when first confronted on a stage in front...front so the interactions are better. Have a good microphone system ready for each panelist if the room needs...what happens to the attention of the audience – phones come out, postures shift, they are checking out...

... wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to... coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000). Chromoproteins also enhance... (2000) Fluorescent pigments in corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038...

...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...Easy to host guests remotely. Easy access from smartphones with low data requirements.  No visuals. Variety...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

... oligonucleotides called morpholinos. When injected into zygotes, morpholinos complementary to a target...activity and does not work easily in zebrafish.) Morpholinos are a convenient and fast way to deactivate genes...whether a gene is deactivated via mutation or morpholino. This observation unlocked novel research into...

...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

...effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and helper...consistently shown promise in reducing leukemia and lymphoma and are in their clinical trial phase. In all ... this results in frequent physiological and morphological changes. Tumors differ in their genotypic and...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase...

...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...

...often used to monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow... Rosella Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources...

...unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine (PE) and recruited to autophagasomal membranes...Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology...protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources...

...inside cells that are on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating...and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application... functional in vivo imaging of neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773...

...steady stream of papers describing new photosynthetic or photosensory molecules that come from basically ...would be helpful to have molecules with better two-photon properties, so you could activate single cells ...

...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...