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We narrowed to 273 results for: plat

Showing: 81 - 100 of 273 results

  1. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...Nobody’s perfect! This is a platitude I’m sure we have all heard. It’s a truth that extends to both people...sequence and assemble using the Illumina MiSeq platform, such as GC-rich regions and highly repetitive...Plasmidsaurus for long-read nanopore sequencing. This platform sequences the entire plasmid in one read and thus...

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ... 2014). The second was an in vitro paper-based platform that allowed gene circuits to be deployed out ... had been working on advancing the paper-based platform toward a more field-ready format when the Zika...we say the timeline to develop our paper-based platform into clinically relevant diagnostics for the Zika...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...that grew on a selective plate versus the number that grew on a non-selective plate. Most bacteria tested...glmS gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for...

  4. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...liquid media or solid media (on plates). In general, amplification on plates can help to ensure that diversity...library varies significantly in plasmid size). On plates, each transformed bacteria will be its own colony...

  5. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...double-stranded break. HDR copies the sequence from a repair template with flanking sequence homology for error-free...systems, and NHEJ will be favored even if a repair template is present. HDR also presents a cloning dilemma...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...

  6. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... to guides in the Broad’s Genomic Perturbation Platform Avana library (used by the Cancer Dependency Map...and Adam Brown from Broad's Genetic Perturbation Platform to design a new library that is agnostic to ancestry... are working with Broad's Genetic Perturbation Platform to modify the CRISpick algorithm to prevent guides...

  7. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ... connect the imaging community and to create a platform for discussions and potential collaborations. ...loads of opportunities to create communities, platforms, and to be a part of projects that connect people...realize. Being at a university provides the perfect platform for practicing initiative and for training in ...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...help us work in large formats, such as 96-well microplates, and thanks to our dedicated product and development...pipeline. After reviewing and testing several platforms for engineering pipelines, we selected Nextflow...workflow would handle plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...complex edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing...potential to reduce negative side effects. This platform offers a promising approach for experimental therapies...

  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    .../L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing glucose and L-arabinose at 37oC for ... arabinose and colonies on arabinose-containing plates will be visibly white, and gas vesicles in cells...from left to right: colony whiteness on inducer plates, white inclusions in cells with GVs via phase contrast...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...general, technical replicates can be thought of as "plate controls". They are NOT independent and are typically...transfection of multiple separate wells (within the same plate) with purified plasmid from the same aliquot/preparation... the same source and were incubated on the same plate at the same time. Although the replicate control...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...phenotype. Incubating the phage display library with plate-bound antigen helps select for high affinity scFvs...bacteriophages. This library is incubating with plate-bound antigen to select for scFvs with high affinity...antibodies  Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...

  13. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...the Director of R&D in the Genetic Perturbation Platform at the Broad Institute and has worked with many...

  14. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...repaired by homologous recombination with a repair template.  Each zinc finger recognizes a nucleotide triplet...advertisement of the technology on various non-scientific platforms, CRISPR has also transcended into the world of... and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing RNA),...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...charged as they pass through a metal deflection plate. Each droplet contains a single cell that is assessed...for a desired parameter is collected in a tube or plate, while a droplet containing a negative cell is discarded.... Many FACS sorters are compatible with 96-well plates as collection vessels and can sort single cells...

  17. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...asked the individuals behind some of these new platforms what their motivations were for developing these...research in their field including datasets and platforms for discussing new developments, and more. As ...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...directed repair templates (see the supplementary info from the paper for details on repair template design). ... Methods 2012. Mar 11;9(5):493-8. MXS cloning platform for imaging and flow cytometry applications Designing...relatively easy to design constructs and repair templates to insert TAP tag fusions directly into the genome...

  19. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...

  20. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...
Showing: 81 - 100 of 273 results