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In Category

We narrowed to 310 results for: reporter

Showing: 81 - 100 of 310 results

  1. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...pathway is ubiquitous, with successful recombination reported in all laboratory E. coli strains tested so far...use of this pathway as a cloning tool was first reported over 25 years ago (Douglas and Howard, 1991, Bubeck...exploiting bacterial in vivo assembly." Scientific reports 6 (2016): 27459. PubMed PMID: 27264908. PubMed ...

  2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring...help you make better fluorescent fusion protein reporters for your studies and greatly lower your chances...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...

  3. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

  4. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...did you use? For animal subjects it is helpful to report the genetic background and catalog number or RRID...take negative data, which is equally important to report. We recently published a paper in Frontiers in ...

  6. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...

  8. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived... 2014). A further development of this approach reported in 2017 uses the direct injection of a CRISPR/...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ...

  10. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not...

  11. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence upon cleavage. Upon ... results in the trans cleavage of the ssDNA-FQ reporter resulting in fluorescence. Cas12a binds all the...results in the continuous cleavage of the ssDNA-FQ reporter. The resulting fluorescence can be observed under...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...primary antibody respectively. Avidin bound to a reporter is then added to the reaction for easy detection... experiments. During detection, a biotinylated reporter enzyme that is pre-incubated with free avidin ...protein of interest leading to amplification of the reporter signal and easier detection. This strategy is ...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...

  16. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ... expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...

  17. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...

  18. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...
Showing: 81 - 100 of 310 results