We narrowed to 106 results for: sacs

1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, Doudna JA (2020...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org...

3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...classic rhodamines and their analogs without sacrificing their small size and membrane permeability (Figure...

4. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...

5. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...

6. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Oct. 14, 2015, 2:30 p.m.

   ...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...

7. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual conference all foster mentorship in different...

8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   Published

   May 2, 2019, 12:41 p.m.

   ...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) -  ...

9. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant...

10. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently...

11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ... only game in town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated...

12. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...can be turned on or off depending on what tet transactivator is used. UAS General expression mRNA Yeast...

13. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology...

14. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...Forward Design: We produced a video celebrating Massachusetts Nonprofit Day, which was the perfect opportunity...

15. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ... et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively. Although each...

16. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ...early investigators making unnecessary short term sacrifices that hurt their goals, rather than focusing on...

17. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Response against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated...

18. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...most widely-used Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered variants...

19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology, Boston University, ...

20. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...presenting your research at conferences such as SACNAS and ABRCMS. These conferences have a lot of programming...