We narrowed to 100 results for: sag

1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...biology and CRISPR genome engineering technologies. Message or follow him on twitter @Vopinator.     References...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also theoretically...

3. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...casual request via email, in person, or instant messaging but we as scientists often prefer data. At Addgene...

4. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   .... No, we’re not all BFFs. Yes, sometimes teams disagree. Yes, sometimes work is a bummer when you’d rather...

5. Negotiating Work and Life: How to Find the Joy

   Type

   Blog Post

   Published

   March 20, 2014, 3:04 p.m.

   ...vacation alone with your partner – no kids. Get a massage – no guilt. Have recreational time with your friends...

6. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   Published

   Nov. 15, 2017, 3 p.m.

   ...each lot of AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’...

7. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...off-target editing, and, for RNA editing, fine-tuning dosage so that enough wild-type MECP2 is made inside the...

8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   Published

   May 2, 2019, 12:41 p.m.

   ...to make a polygamous species monogamous. Anolis sagrei (Doug Menke) No methods were available to create...

9. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ...counting, or sequence assembly), but the order and usage of the scripts needs to be customized in the pipeline...

10. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...stained with Anti-PSD-95 [K28/43R] (70 µm mounted sagittal section). Credit: Zhuhao Wu, Weill Cornell Medicine...

11. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...results in the pooled library lentiviral prep. B) Usage of a pooled library lentiviral prep for experiments...

12. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ... online video market ever since. The take home message of all of this appears to be that video is the ...

13. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ...protein folding, and deal with sub-optimal codon usage (Table 1) A few mutations are common to all or most...

14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ... larger cells. Note, we don’t mind the warning message at the top, as our cells of interest do not lie...

15. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ... to a chalk talk is to effectively convey your message. Key Things to Think about When Developing your...

16. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    Published

    Aug. 23, 2016, 2:30 p.m.

    ...slides and how the slides complement the spoken message. Learn from their level of explanation in presenting...

17. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...achieve success in their careers. The take-away message I took from the career panel was that there are...

18. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    Published

    Dec. 20, 2022, 2:15 p.m.

    ...expression of that gene in cells that also express GAL4. Usage of this system revolutionized genetic studies in...

19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...clones were bi-allelic in our hands). Take-home message In our hands, delivering Cas9 protein in complex...

20. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max with a 4.2-6-...