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...Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila...Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists to study...

...positive effect on cancer treatment (Ref 1). As described in the presentation, starvation or the use of ... with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel...

... NEB's Resource describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli...

...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ...

... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other...

...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

...Cell, Musunuru, Chad Cowan and their colleagues describe a much more efficient tool for doing that disease...people unfamiliar with this new TALEN kit, can you describe it for us? Musunuru: TALENs are a type of genome...

...features with optimal biological performance are described as Click Biology (Howarth, 2025). Figure...  Click Into Biological Building Click Biology describes the search for robust, reliable reactions, using...

...locus-specific knock-in of FKBP12F36V. Detailed protocols describing the use of these plasmids are available here....degradation, and lead author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet...

...read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this post we ...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...

...Systems Biology in November, Voigt and Alex Nielsen describe what’s possible when multi-input CRISPR/Cas genetic...many deposits to Addgene over the years. Can you describe the toolbox you are putting together?  Voigt: ...

...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

... in red in the spreadsheet (Figure 2) and are described in Table 1. Some cells have drop-down options ... Primary Vector Type The vector type should describe how your plasmid is intended to be used. Choose...

...A recent publication [1] from the Sánchez lab describes the plasmids and protocols required for both WISH... which are available through the deposit) are described in the Sánchez lab publication [1] so that the...

...soCoChR-expressing system. Taken together, Shemesh et. al. describe a novel system for the precise mapping of neuronal...the single-cell level. Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid...

... coming, right? Non-coding RNAs (ncRNAs) are transcribed but do not encode proteins. They play important...sequence can completely disrupt expression of the transcribed ncRNA (Jiang et al. 2014).  Simple knock-out ...

...efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017), they modified...undesired off-target editing effects. ABEs have been described to introduce base changes in cellular DNA and ...

...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

...synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated...template of choice and allowing these cells to transcribe, translate and modify your protein of interest...

...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...membrane, a concern with several of the previously described systems. Several adaptations of this method have...