Skip to main content
Addgene
Showing: 81 - 100 of 306 results

  3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ...

  4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other...

  5. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...Cell, Musunuru, Chad Cowan and their colleagues describe a much more efficient tool for doing that disease...people unfamiliar with this new TALEN kit, can you describe it for us? Musunuru: TALENs are a type of genome...

  7. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this post we ...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...

  8. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...locus-specific knock-in of FKBP12F36V. Detailed protocols describing the use of these plasmids are available here....degradation, and lead author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet...

  10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ... coming, right? Non-coding RNAs (ncRNAs) are transcribed but do not encode proteins. They play important...sequence can completely disrupt expression of the transcribed ncRNA (Jiang et al. 2014).  Simple knock-out ...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation of the...FRET probe, try searching PubMed for articles describing the FRET tool that you are looking for and check...

  15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... in red in the spreadsheet (Figure 2) and are described in Table 1. Some cells have drop-down options ... Primary Vector Type The vector type should describe how your plasmid is intended to be used. Choose...

  16. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...component as well as a small molecule which we will describe in more detail below. Types of Voltage Indicators...membrane, a concern with several of the previously described systems. Several adaptations of this method have...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated...template of choice and allowing these cells to transcribe, translate and modify your protein of interest...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

  19. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

  20. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...their BioRxiv post, the authors generated and described several improved genetically encoded fluorescent...Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion...
Showing: 81 - 100 of 306 results