We narrowed to 472 results for: shi

...mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength from...FPs, Stokes shift is less than 50nm (often much less).  For LSS proteins, the Stokes shift is ≥ 100nm....

...chromophore concealed at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed...intensity and photostability of GFP. This also shifted its major excitation peak from 395 nm to 488 nm...S65T Increased fluorescence, photostability, and a shift of the major excitation peak to 488 nm F64L Increased...phenol component (cyan derivatives) Y66H Blue-shifts the wavelength (blue derivatives) Y145F Increases... aggregation at high concentrations T203Y Red-shifts the wavelength (yellow derivatives) A206K Interferes...

...careers, women in STEM, work-life negotiation, and mentorship, we decided to have her participate in an AMA...careers, women in STEM, work-life negotiation, and mentorship. We can direct people to the AMA in the future...whole point is to meet actual people to build relationships. I also like Twitter because I can get a little...important to find good mentors and incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of... mentorship...

...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...characterize this phenomenon and found a general relationship between the distance of a mutation from the ...(and let them cut close).   Figure 2: Relationship between cut site to mutation distance and editing...mutations When thinking further about the distance relationship described above, we realized that, in addition...incorporation efficiency, we might exploit this relationship to direct the zygosity of the cells. This was...

...Chapter 1 - Introduction to Mark Springel, Olivia Ho-Shing, and the Journal of Emerging Investigators. Chapter...Chapter 3 - JEI target audience and their current readership. Encouraging students to do science anywhere.... building curricula and developing one-on-one mentorship programs. Chapter 6 and Outro - Interview with...the future. Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian...

... colocalization factor to assess the spatial relationship between the targets. Or, it might be sufficient...non-specifically – try optimizing your blocking and washing conditions or consider using a different antibody...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order to ensure...getting started with two or three targets or you’re pushing the limits like Addgene depositor Ronald Germain... Speranza, E., Chu, C. J., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat...

... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...too, they may not have had experience writing, publishing, or discussing career opportunities with the ...PI removed a woman from the appropriate first authorship position on a publication of her project and ...reminders of blunders, setting target up to fail, shifting goal posts without telling the target Warning...open PI helps lab members determine project "ownership" and author order PI lets lab members fight it...

...located.  The export license, if granted, allows us to ship rabies plasmids and viral vectors containing genetic...control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences from... Once the application is approved, Addgene then ships the material following our normal process.   Due...have any questions about this or other Addgene shipping and ordering processes, don’t hesitate to contact...

... scientist. Leadership and management Take a lead from the amazing students at Washington University who...You are finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You ...in your department, create them and show your leadership skills too. Teach classes Go to workshops and...

...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid... 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different...cells into pluripotent stem cells. Plasmids from Shinya Yamanaka's lab and James Thomson's lab were the...

...https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology ..., Demircioglu, F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E....j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. ... S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng...

...absorbed. This phenomenon is known as the Stokes Shift, or the difference in wavelength between peak excitation...background signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...accurate and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower...for intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their..., 987–994. https://doi.org/10.1038/nmeth.4403  Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H.,...

..., hypercompetition is corroding values, we are shifting away from PI driven basic research, there is resistance...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...systemic flaws” by Bruce Alberts, Marc W. Kirschner, Shirley Tilghmanc and Harold Varmus     ...

... Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address...details on what they accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a .... They’re really easy to spot and even easier to ship back. Be that person! If you use serological pipettes...in your tissue culture room for the wrappers and ship them back. Start a glove recycling program at ...

...communication. When I started my Science Communication Internship with Addgene, I didn’t know a lot about scicomm... few Addgene guest blog pieces. Throughout my internship, my interest in scicomm has grown and now it ...is Hans Packer - science writer  When Hans was finishing up his PhD in Molecular Neuroscience at the University...Technologies (IDT). “It was fairly accidental. I was finishing graduate school, and didn’t have anything lined...

...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ...manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide...to sign up for a writing intensive weekend in Washington D.C. at the ASM headquarters. I decided to go...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

...islands,” said Tetsushi Sakuma, a lecturer at Hiroshima University who works with Takashi Yamamoto. “Addgene...materials at Addgene is that we cover all of the shipping logistics and facilitate all material transfer...

...Waardenburg Syndrome Last but not least, Meng-Shin Shiao (right) of Mahidol University’s Ramathibodi Hospital... Addgene will make a substantial difference in pushing the work forward. Xeroderma Pigmentosum Hendrik...precisely.  To put this award into perspective, Shiao wrote in an email: “In Thailand, our annual operating...

...infrastructure and offered to collaborate with them. The partnership was fruitful and a first version of the study... the power of collaboration and join efforts to shift scientific culture towards a more generous and rewarding...asymmetric collaborations.” These are transient partnerships where a researcher makes a small but crucial...from becoming starting points for long-lasting partnerships Join us! We are a community of researchers who...

...which can help determine structure and function relationships or, in more practical applications, allow a ...some systems, rare codons and four-amino acid frameshift codons are also used. Note that some systems ...idiosyncrasies presents a significant barrier for those wishing to use it as a tool. In short, while the technical... any of a huge variety of ncAAs to study the relationship between structure and function…if you can get...