We narrowed to 291 results for: tac

...might wish to include are a references section, contact information, and potentially a photograph of yourself...section where you can insert your references and contact information, and it is generally acceptable for...

... for a knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces...use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1...

... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...

...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...

...diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR technology ...systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies have taken...

...that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors undergo...solution can be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction...

...PBS (Phosphate Buffered Saline), that comes in contact with the cells should already be at 37 °C. Most.... If they reach confluence, they stop growing (contact inhibition) and it takes them time to recover after...

...events plays itself out: The researcher breaks eye-contact with any other human in the room. Their eyes are...Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if we could make a model of ...

...cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody...Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell ...

...libraries may not have amplification protocols attached.  Final step: validation  Remember, there is ...your library amplification protocol, feel free to contact us at [email protected]. Happy amplifying! More...

...reducing the time it takes to image through a large z-stack (8).  One limitation of LSFMs using single side ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

...on fluorescent proteins and their applications, tackles much broader issues in this brief volume. He examines... Watsons.” In Part Four, “New Science,” Zimmer tackles more recent examples of scientific advances, and...

...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...epitope tags into single FP scaffolds with either intact or darkened chromophores. These probes are both...

...CRISPR analysis tool is part of Synthego’s full stack Genome Engineering Solutions, which also includes...editing with the CRISPR Gene Knockout Kit. Please contact us at [email protected] if you have any questions...

...5: Colony Screening Use Primer 92F (5′-cattacgcaattggacgacaac-3′) and Oligo 2 (specific to your target.... If you have specific questions, feel free to contact the Molla Lab (X account: @Kutub_joy). Dr. Kutubuddin...

...the facility that produced the viral vector lot. Contact us to determine which facility produced your viral...experiments were performed on your lot, please contact us. Full sequencing of the Viral Genome Next-generation...

... write a blog post, edit other blog posts, and contact PIs for an upcoming visit to a university. My to-do... of previous depositors from Boston University Contact Dr. Gilmore directly to see if he knows anyone ...

...the classical DSBR pathway, the 3’ ends invade an intact homologous template to serve as a primer for DNA...continue to cleave DNA if the gRNA/PAM site remains intact. In other words, Cas9 will keep cutting and repairing...

...how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and...ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these methods...

...for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1) layer...cells out of all BiPVe3-dTom cells. C) Confocal stack of a biocytin-filled cell expressing BiPVe3-dTomato...