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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 289 results for: tac

Showing: 81 - 100 of 289 results

  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... for a knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces...use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1...

  2. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...

  4. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...

  5. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR technology ...systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies have taken...

  6. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors undergo...solution can be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction...

  7. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody...Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell ...

  8. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...PBS (Phosphate Buffered Saline), that comes in contact with the cells should already be at 37 °C. Most.... If they reach confluence, they stop growing (contact inhibition) and it takes them time to recover after...

  9. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...libraries may not have amplification protocols attached.  Final step: validation  Remember, there is ...your library amplification protocol, feel free to contact us at [email protected]. Happy amplifying! More...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...events plays itself out: The researcher breaks eye-contact with any other human in the room. Their eyes are...Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if we could make a model of ...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...reducing the time it takes to image through a large z-stack (8).  One limitation of LSFMs using single side ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

  12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...on fluorescent proteins and their applications, tackles much broader issues in this brief volume. He examines... Watsons.” In Part Four, “New Science,” Zimmer tackles more recent examples of scientific advances, and...

  13. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...epitope tags into single FP scaffolds with either intact or darkened chromophores. These probes are both...

  15. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...the facility that produced the viral vector lot. Contact us to determine which facility produced your viral...experiments were performed on your lot, please contact us. Full sequencing of the Viral Genome Next-generation...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...the classical DSBR pathway, the 3’ ends invade an intact homologous template to serve as a primer for DNA...continue to cleave DNA if the gRNA/PAM site remains intact. In other words, Cas9 will keep cutting and repairing...

  18. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ... write a blog post, edit other blog posts, and contact PIs for an upcoming visit to a university. My to-do... of previous depositors from Boston University Contact Dr. Gilmore directly to see if he knows anyone ...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and...ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these methods...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...Theoretically, as long as all the plasmid features remain intact, plasmid multimers function just as well as monomers...multimers? Let us know in the comments below or contact us at [email protected]. Resources and References...
Showing: 81 - 100 of 289 results