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We narrowed to 693 results for: ust

Showing: 81 - 100 of 693 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...their antibiotics   Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic... familiar?). Carb is degraded by beta-lactamase, just like amp is. Thus, the AmpR gene confers resistance...and eukaryotic selection, whereas other plasmids must carry both a bacterial and a mammalian antibiotic...="16" purpose="decorative" title="Check icon" %} Just bacteria {% icon icon_set="fontawesome-5.14.0" name...title="Check icon" %} Spectinomycin Excellent No Just bacteria Not SHuffle cells {% icon icon_set="fontawesome...

  2. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right...and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA ...terminators can serve to interrupt genes (or gene clusters) in metabolic engineering. So far, we’ve used ...that is 2.6 times higher when compared to the industrial strain. In our metabolic engineering case study...high-yielding strain which had 6 times the yield of the industrial strain. Not only is MUCICAT better, it is faster...engineering with improved cargo dedicated to gene (cluster) interruption.     Plasmids for each of these...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...workflow management system As novices to creating a robust data pipeline that would be reliable and available...chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...the order and usage of the scripts needs to be customized in the pipeline for the specific needs of the...Since each process can use a unique container, we customized each container for its particular purpose and... a complete pipeline however, required more than just identifying criteria that we needed. Prior to using...Nextflow script. With our completed pipeline, we could adjust the compute resources when needed, for faster runtime...

  4. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...immediately and some of them may just take some getting used to. Just remember: change is good. You ...job is defined as sitting down at a computer and just creating, which for many scientists is the most ... job” could entail any number of career paths--industry, nonprofit organization, law office, journal editor...and interact with...non-scientists! Maybe you’ve just spent 5-10 years learning to “speak science,” and...approach that transition dreading the change, the adjustment period will be that much longer. If you approach...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...connectivity to open source data repositories. To illustrate this, let’s take a look at the RSpace ELN.  The... commonly used online file storage tools, as illustrated in this video, and to data stored on institutional...servers. Connectivity to other tools used in research Just as researchers need to access and reference external...fully supported API that enables development of custom integrations with other tools and resources. As...is the integration between RSpace and Slack, illustrated in this video. This is a two-way integration....protocols.io, the protocol sharing repository. As illustrated in this video the integration enables RSpace ...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... blue light CRY2-CIB1 heterodimerizes or forms clusters under blue light Check out our Optogenetics Guide...specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID along... One great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of ...Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted from Aljiboury et al., 2023, under...earlier work on Rab11, the team used optogenetic clustering of Rab8, Rab11, and Rab35 to understand their...2022, bioRxiv, under a CC-BY-NC-ND 4.0 license.   Just make sure you don’t overdo it! The moderately-enhanced...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...popularize our kit resources,  we’re highlighting just a few of the many useful kits you can order from...the transcription terminators in this series are just the tool you need. The plasmids in this portion ...6803." Biology open (2016): bio-017129. Hsia, Justin, et al. "Modular Synthetic Inverters from Zinc ...one-pot reaction. Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil...Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor to down-regulate...Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature communications...

  8. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... can add an EcoRI site just 5’ of the start of the gene and a HindIII site just 3’ of the end of the gene...carefully choose your backbone and insert - these both must have compatible cut sites for restriction enzymes..., you would want the start of the gene to end up just downstream of the promoter found in the backbone...ends will anneal leaving the 5’ end of your gene just downstream of the promoter and placing the gene ... your insert and vector, unfortunately you can’t just throw the digestion mixtures together. You need ...

  9. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    .... The power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific...offering you unparalleled flexibility. But this is just the beginning. We’re working toward giving you more... of this incredible journey. Together, we're not just sharing research materials — we're accelerating ... D., Cooper, J. A., Crosby, K., Harvey, K. J., Houston, D. W., Kumaran, R., Rego, M., Schofield, C., …...024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A....

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...freezer… you get the idea. What if there were a way to just make one fusion protein that switches to any color...linked to whatever molecule you choose (Figure 1). Just express the fusion protein and add a specific ligand..., 1998), this 15 amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the results... a fluorescent protein for you. And some ligands just aren’t going to work in certain applications — the...and ease of use. With so many possibilities from just one or a few constructs, it’s no wonder that self-labeling...

  11. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...group is different in a certain direction (i.e., you just want to measure if a sample is greater than a control...additional tests, referred to as post-hoc analysis, must be performed. These will be discussed later. One... want this information, multiple ANOVAs/ANCOVASs must be performed first, and then post hoc tests can ...this case, save yourself from family-wise error and just perform the relevant comparisons using Dunnett’s...beyond the ones detailed in this article. These are just some of the most common parametric comparison tests...mathematician to select the right statistical test, just do your homework and happy testing!   References...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...next career stage (ex: grad school, med school, industry) Other roles you might see Lab manager: ...what I wanted to do with myself when I was 18. I just knew I was interested in trying research, and if...mistakes. Ultimately, though, scientists are all just people. I’ve found that in the right environment...making an account, following interesting people, and just lurked online for a while and observed. Apply for...thinking that I had to pick a few things to do and just stick with them for the next four years. But college...not limited to working at a bench in academia or industry, so feel free to explore! Addgene has an entire...

  13. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...position to another throughout your career. Plus, just one role can encompass many of these activities ...so diverse, you can do it in so many places-- not just the government. When you talk to folks about science...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...and your next steps. This advice is important, but must be taken through your own personal lens.  For me...define your why. How do you do science policy? Just like there are many different types of science policy...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

  14. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... PhDs Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple...research. Why serendipity matters Serendipity is not just a stroke of luck; it's a mindset that can lead to...successful research career, whether within academia or industry. Albert Einstein once said: “There are only two...leave a little space for the unexpected — it might just be the key to unlocking the next frontier of knowledge...

  15. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...filters to limit results to people that work in industries you’re interested in (i.e. biotechnology, pharmaceuticals...switch your field of work, both in academia and in industry. Advancing along a career path in these two fields...fields looks different however. In industry the career ladder is larger and there are more opportunities...Career advancement for PhDs, both in academia and industry, often means moving from the lab to a desk, although...PhD, but if I had to do grad school over, I would just do the master’s! If you'd like more career development...

  16. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...the words and actions of their managers. You can’t just think it, you have to tell them. A good manager ...consultation, advice and brainstorming. Employees must understand the part they play in the goals of the...harder to manage when the measure of work is not just time spent at the desk, but it is worth it. This...focus on deliverables. It does not mean a manager must allow his team to work mostly at home. Having a ... solve (I think this is true of most people, not just scientists) Are self-directed, but need precise ...

  17. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...the shakerTurn the LEDs onAnd when you wantYou can just turn them offNo need for any washNo need for any...about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich. “Instead of a graphical...transcription with the flick of a light switch and shuts off just as quickly. The original AraC system, which uses...exposed will fluoresce. With these images, you can see just how spatially precise the activation is.   ...Romano et al., 2021 with permission.   BLADE is just one of the several optogenetic tools that Di Ventura...

  18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ..., which enables insertion of custom genomic modifications, is very robust in C. elegans, and the methods...transcriptional reporter, and loss-of-function allele in just one injection step! The entire protocol takes about...Cas9 so successful in this species, namely, the robust HDR mentioned above, an accessible germline, and...Figure 2: Schematic from Dickinson et al. (2015) illustrating the steps to fluorescently tag a C. elegans ...

  19. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...RNA-seq clustering of cells transduced with the TF pooled library. Louvain community clusters of individual... system. TF screens can be designed to identify custom TF programs to acutely initiate disease onset, ...reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation protocol...s something you’d like that’s not yet available, just ask an Addgene scientist at [email protected]! References...

  20. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...they thought would be moving forward instead trying just to get going. It’s like being stuck in jail in Monopoly—you...you did and build on it going forward. This is not just a question of being honest and straightforward, ...the one that you need to spend the least time on—just put down a basic outline and refer back to the original...thought was a problem in fact was not, and it was just that they were doing something differently. Communication...’ll have fewer students and postdocs sitting frustratedly in reproducibility jail and more effective collaboration...
Showing: 81 - 100 of 693 results