We narrowed to 101 results for: yeast

Should you codon optimize your gene or not? Here, we discuss several reasons for and against codon optimization.

Nov. 12, 2020, 2:15 p.m.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

Aug. 27, 2020, 1:15 p.m.

Acoustic reporter genes (ARGs) are activated by ultrasound instead of light. Updated bacterial and mammalians ARGs can provide contrast and improved utility.

Jan. 24, 2023, 2:15 p.m.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Oct. 13, 2022, 1:45 p.m.

Learn more about unique organisms that are being developed as model systems in this blog post.

May 2, 2019, 12:41 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Oct. 19, 2017, 1:54 p.m.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

April 7, 2016, 2:30 p.m.

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

June 3, 2021, 1:15 p.m.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

April 9, 2019, 1:13 p.m.

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

Nov. 15, 2018, 1:50 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Learn about a useful technique to screen phage genomes for host binding proteins and discover new research avenues.

Sept. 29, 2016, 2:30 p.m.

Learn what non-model organisms are, why scientists use them, and what they can teach us.

July 21, 2026, 1:15 p.m.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

July 6, 2021, 1:28 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

Get perspectives and possibilities for future action from scientists and other citizens who participated in the March for Science.

Oct. 24, 2017, 1:54 p.m.

Learn about new tools in Addgene's collection, including new prime editing and CRISPR applications, tools for neuroscience research, and more!

Sept. 1, 2026, 1:15 p.m.

Learn about AlphaFold and how you can use it for protein structure predictions to inform interesting research questions.

Sept. 29, 2026, 1:15 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.