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We narrowed to 803 results for: Cre

Showing: 491 - 500 of 803 results
  1. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...expense and requirement for lab equipment. Plus they create plastic waste. Kits can also have the annoying ...and filters can be stacked together in series to increase binding capacity. Figure 1: Purify DNA using...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...
  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...a double-edged sword. An antibody’s large size creates more distance between the target antigen and the...also allows for better tissue penetration and decreases the distance between a fluorescent tag and the...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...
  3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...overexpression of protein fusions to gene-edited cells can decrease the number of fluorophores, compromising the photon...improved fluorophores are making chemical dyes increasingly attractive and accessible to cell biologists—and...fluorogenicity, photoactivation etc.—can further increase the photon budget and allow us to push further...
  4. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...that can function in more than one host species. Created with BioRender.com. How are shuttle vectors...viral oris to drive amplification, which helps increase cellular uptake, but this approach requires compatible...eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular language...
  5. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...proteins cooperatively can separate the DNA strands. Created with BioRender.com. Once DnaA breaks the local...helicases to continue unwinding the strands. This creates two replication forks that move in opposite directions...pseudoknot from folding, blocking replication. Created with BioRender.com. Plasmids under stringent...
  6. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...researchers in the field. Finally, you can sound credible and cool when you casually mention “Haery et al... al., showed that MYC expression was increased…” when discussing the review topic with your peers. Start...will also paraphrase what I have heard many other creative people say about writing: you don’t know what ...
  7. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...prognoses. Survival rates for mesothelioma are creeping upwards, and while most people still die within...world. New mesothelioma cases could be greatly decreased through a ban on asbestos. Nonprofits like the...Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources on Addgene.org ...
  8. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...expression transposases leading to increased integration frequency. (C) Increasing rounds of transfer on transposition-inducing...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...
  9. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...and multiple gRNAs into separate AAV vectors, increasing overall packaging capacity but necessitating ...efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct using ...histologically normal and liver injury markers were not increased compared to a control AAV-GFP. Not only did the...
  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...1998). As the copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit ...inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...
Showing: 491 - 500 of 803 results