We narrowed to 904 results for: CAN

...above can be made for expressing your favorite T-cell receptor, or perhaps any other tool that can benefit...articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral...different epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to... Fixation, chemical staining, and light exposure can negatively affect FPs during these processes. The...with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents...

...genetic drift, scientists can freeze hybridoma cells before culturing or they can clone the genes from the...When you are originally selecting a hybridoma, you can choose one that produces high-affinity/high-avidity...time as the hybridoma cells divide. These changes can alter the antibody that is actually produced by the...antibodies produced by the hybridoma. Any genetic drive can be identified by sequencing the heavy and light chains...myeloma cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive...you’ll get a false negative signal - the antibody can’t recognize the protein even though it is present ...

...also not suitable for spatially limited studies and can fluctuate with changing culture conditions. The use...of these problems; inexpensive tools such as LEDs can be engineered to deliver precise, consistent, and...Complicated patterns of light intensity and wavelength can be used to study dynamic processes. Conversely, varied... TCS activated by green light; these two systems can be coexpressed for sophisticated control of gene ...dynamic range of these systems ensures that they can be used with many types of proteins, including Cas9...

...look like... when it looks good, that’s a high that can last for several days.” Working with CRISPR Recently... genes are involved in biological process X, you can do with CRISPR technology...” and “with CRISPR, just...specific edits to the genome. Such characterization can be done in a low throughput way now, but Doench says...screening) such that thousands of individual edits can be studied quickly. Involvement with Addgene Although...] success and your success moving forward.” You can give Dr. Doench your own welcome in the comments ...worked on using RNAi screens to learn more about cancer biology in Ed Harlow’s group at Harvard Medical...

...techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts here. Now...covered antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening...expression). When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5...small fragment is inserted in-frame, read-through can lead to a functional β-galactosidase enzyme and a...take up the α-complementation plasmid. This way you can quickly and easily identify colonies that not only...-white screening is a good way to narrow down candidates for more specific analysis, like PCR or restriction...substrate in defined medium, without which the cells cannot reproduce to form colonies (used to complement ...

... Choosing a lab can be a major decision. A science trainee will spend 4-7 years working for one person...not be the driving factor. The following questions can serve as a guide for the quest of a perfect "fit"...this is one case where the topic of your research can make your transition easier – choose a lab that works...goal is to spend as much time in the "zone" as you can. You know you are in the zone when you lose track...always be in the zone, but even brief stays there can make all the difference. Choose a lab that brings...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...also includes a useful list of questions for a candidate to ask lab heads, lab members and lab neighbors...

...plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research...is fairly straightforward and the resultant cells can either express the plasmid DNA transiently (similar... to the cell, is expressed from the plasmid, and can be assayed conveniently. A direct test for your gene...selection; however, negative selection techniques can be used in conjunction with positive selection to...for mammalian expression – instead use G418. This can be confusing since the neo/kan gene confers G418 ...

...to avoid handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant females...Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either...Alternatively, the gRNA, Cas9, and donor oligonucleotide can be electroporated into the mouse zygote (Qin et al...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through... an oligonucleotide or plasmid), precise changes can be made in the genome via the homology directed repair...from the fact that CRISPR is so efficient that you can inject the reagents directly into fertilized mouse...compared to more conventional methods. First, one can work with almost any strains of mice, as compared...

...specific type of neuron in a specific brain region can result in a particular neurotransmitter release or...tools required for each one? Behavioral Optogenetics can be broken down broadly into three main steps: 1)...about the many different opsin variants and how they can be used is to consult the many primary research papers...neuronal subtype. However, the length of promoter that can be used in an AAV construct is limited to only a ... stereotaxic surgery (stereotaxic surgery videos can be found on youtube [above] and jove). Delivery of...frame with a digital readout. The advantage is you can “zero” your coordinates at bregma which allows you...protocols on stereotaxic surgeries for viral injections can be found here [5, 6]. Opsin transgenic mice Transgenic...

...and the mice. While not an exhaustive list, these can include what’s being injected, the type of experiment... and restraint method. Many different substances can be injected, including cells, drugs, DNA, viral vectors...to these as “tail vein injections.” However, they can also be given retro-orbitally, behind the eye. IV...technique, as it requires intubation of the mice, which can be tricky given their small size. This technique ...nose, they also pass through the upper airways and can target the airways before the lungs. This is most...tears or punctures to the throat. The feeding tube can be flexible or metal, depending on researcher preferences...