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We narrowed to 892 results for: CAN

Showing: 501 - 510 of 892 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... Choosing a lab can be a major decision. A science trainee will spend 4-7 years working for one person...not be the driving factor. The following questions can serve as a guide for the quest of a perfect "fit"...this is one case where the topic of your research can make your transition easier – choose a lab that works...goal is to spend as much time in the "zone" as you can. You know you are in the zone when you lose track...always be in the zone, but even brief stays there can make all the difference. Choose a lab that brings...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...also includes a useful list of questions for a candidate to ask lab heads, lab members and lab neighbors...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research...is fairly straightforward and the resultant cells can either express the plasmid DNA transiently (similar... to the cell, is expressed from the plasmid, and can be assayed conveniently. A direct test for your gene...selection; however, negative selection techniques can be used in conjunction with positive selection to...for mammalian expression – instead use G418. This can be confusing since the neo/kan gene confers G418 ...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...to avoid handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant females...Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either...Alternatively, the gRNA, Cas9, and donor oligonucleotide can be electroporated into the mouse zygote (Qin et al...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through... an oligonucleotide or plasmid), precise changes can be made in the genome via the homology directed repair...from the fact that CRISPR is so efficient that you can inject the reagents directly into fertilized mouse...compared to more conventional methods. First, one can work with almost any strains of mice, as compared...

  4. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...protocol can easily be adapted for other types of proteins.    If this video is helpful, you can find our...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...specific type of neuron in a specific brain region can result in a particular neurotransmitter release or...tools required for each one? Behavioral Optogenetics can be broken down broadly into three main steps: 1)...about the many different opsin variants and how they can be used is to consult the many primary research papers...neuronal subtype. However, the length of promoter that can be used in an AAV construct is limited to only a ... stereotaxic surgery (stereotaxic surgery videos can be found on youtube [above] and jove). Delivery of...frame with a digital readout. The advantage is you can “zero” your coordinates at bregma which allows you...protocols on stereotaxic surgeries for viral injections can be found here [5, 6]. Opsin transgenic mice Transgenic...

  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...undomesticated which limits the tools scientists can use to study them. For example, gene knockdown with...into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain, ...selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader range of bacteria. Once...ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes, like Mobile-CRISPRi. The ...understanding the basic biology of bacteria, Mobile-CRISPRi can be used to identify antibiotic resistance genes. .... Key takeaways of Mobile-CRISPRi Mobile-CRISPRi can be used in non-model bacteria species where its modular...PMC6424567. Peters, Joseph E. "Tn7." Mobile DNA III. American Society of Microbiology, 2015. 647-667. PubMed...

  7. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ... For example, you can analyze experiments designed outside CrispyCrunch, or you can input pre-existing...CrispyCrunch While CrispyCrunch may evolve over time, you can depend on it to stick to a few guiding principles...the acceleration of science. It's open-source. You can see all the current code on GitHub, ask us questions...weight (which ranges between 0 and 1) so that they can be used in the rank equation above. Cut-to-insert...between 0 and 1) (shown in the graph below) so that it can be used in the rank equation above. Generally, the...with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello ...completed a MSc in Psychology at McMaster University in Canada. He's happy to be contributing again to science...

  8. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...and the mice. While not an exhaustive list, these can include what’s being injected, the type of experiment... and restraint method. Many different substances can be injected, including cells, drugs, DNA, viral vectors...to these as “tail vein injections.” However, they can also be given retro-orbitally, behind the eye. IV...technique, as it requires intubation of the mice, which can be tricky given their small size. This technique ...nose, they also pass through the upper airways and can target the airways before the lungs. This is most...tears or punctures to the throat. The feeding tube can be flexible or metal, depending on researcher preferences...

  9. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...academic vs. industry postdoc  Time: Academic postdocs can be long—5 years or more, especially in the biological...AstraZeneca, this is three years. This time limit can feel like a lot of pressure. However, it means that...has ties to industry, the drug discovery process can be a bit of a mystery to graduate students in academia...immersed in that environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca...AstraZeneca, you are expected to publish, but this can vary depending on which company you choose. If publishing... In this scenario, finding someone with whom you can have an in-depth discussion about your project might...have less time to explore scientifically since you can’t afford to spend too much time working on something...

  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...Using their PITCh plasmids, GFP knock-in cell lines can be created in about a month and a half, without the...In contrast, the short homology required by MMEJ can easily be added via PCR amplification. Given the ... is a robust method for genomic insertion. PITCh can also be adapted for whole-plasmid integration if ...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important to... of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms where...interesting to see what new editing possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (...
Showing: 501 - 510 of 892 results