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We narrowed to 803 results for: Cre

Showing: 501 - 510 of 803 results
  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...each project. This creates accountability on the part of each developer and creates trust amongst the ... Addgene grows and evolves, we will be here to increase all of our teams’ efficiency and productivity....
  2. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...just might personally hand your credentials to HR, greatly increasing your chances of getting a phone ...can use to better prepare for your career, and increase your chances of landing that next coveted job!...
  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...made use of mazF as a counterselectable marker to create unmarked genetic modifications in the yeast Pichia...counter selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF ...Morin and James Kaper used the hok/sok system to create strains that can stably express antigens in sufficient...
  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...glutathione reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...
  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...you need to speak to start creating slides or thinking about content. Create a Google.slides or Powerpoint...capture interesting key results, striking images, creative ideas, and engaging stories as you encounter or...
  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...percentage of dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin...muscle stem cells are modified by this approach, increasing the possibility that edits will persist long-...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the ...
  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...should be able to apply someone else's new and incredibly useful technique simply by following the abbreviated...49:45 - 1:18:45), there are also initiatives to create more reliable reagents for future research. I’ve...IPI is working to address this issue directly by creating well characterised antibodies against all human...
  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...fluorescent dyes, and fluorescent ligands. Figure created with BioRender.com. Fluorescence refers to...fluorescent protein tags, such as high specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...
  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...interest. By correlating your data sets, you can increase confidence that the labeling observed represents...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...
  10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...is a significant hurdle made worse by an ever increasing marketplace. Since 2013 about 11 tools have been...the user is expected to interact with the tool (a screen shot and overview of the table can be found above...re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile...
Showing: 501 - 510 of 803 results