We narrowed to 904 results for: CAN

...undomesticated which limits the tools scientists can use to study them. For example, gene knockdown with...into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain, ...selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader range of bacteria. Once...ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes, like Mobile-CRISPRi. The ...understanding the basic biology of bacteria, Mobile-CRISPRi can be used to identify antibiotic resistance genes. .... Key takeaways of Mobile-CRISPRi Mobile-CRISPRi can be used in non-model bacteria species where its modular...PMC6424567. Peters, Joseph E. "Tn7." Mobile DNA III. American Society of Microbiology, 2015. 647-667. PubMed...

...academic vs. industry postdoc  Time: Academic postdocs can be long—5 years or more, especially in the biological...AstraZeneca, this is three years. This time limit can feel like a lot of pressure. However, it means that...has ties to industry, the drug discovery process can be a bit of a mystery to graduate students in academia...immersed in that environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca...AstraZeneca, you are expected to publish, but this can vary depending on which company you choose. If publishing... In this scenario, finding someone with whom you can have an in-depth discussion about your project might...have less time to explore scientifically since you can’t afford to spend too much time working on something...

... For example, you can analyze experiments designed outside CrispyCrunch, or you can input pre-existing...CrispyCrunch While CrispyCrunch may evolve over time, you can depend on it to stick to a few guiding principles...the acceleration of science. It's open-source. You can see all the current code on GitHub, ask us questions...weight (which ranges between 0 and 1) so that they can be used in the rank equation above. Cut-to-insert...between 0 and 1) (shown in the graph below) so that it can be used in the rank equation above. Generally, the...with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello ...completed a MSc in Psychology at McMaster University in Canada. He's happy to be contributing again to science...

...Using their PITCh plasmids, GFP knock-in cell lines can be created in about a month and a half, without the...In contrast, the short homology required by MMEJ can easily be added via PCR amplification. Given the ... is a robust method for genomic insertion. PITCh can also be adapted for whole-plasmid integration if ...expressing GFP from a given promoter, and the technique can be adapted to other transgenes. It’s important to... of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms where...interesting to see what new editing possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (...

...functional composition. In other words, these parts can be used over and over again in combination with novel...wants to develop a language for programming life. We can work backwards and say for this to be successful ...still missing? Endy: That's a great question. You can see that we have the beginning of control around ...mostly to the imagination. The question is: what can you use biology to make? If you think about the BIOFAB...someone to forward engineer a system of 10 genes. You can now forward engineer a 10-gene system to do something...to its users and to the design community: If you can begin to reliably put together 10-gene systems, what...

...protocol can easily be adapted for other types of proteins.    If this video is helpful, you can find our...

...Running a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol...share tips for this tricky application.    You can check out all of our protocols on our Protocol page...

...to order plasmids she can use as positive controls in PCR-based cervical cancer screens. This third grant...these screens can then undergo more routine screening allowing for early cervical cancer detection, treatment...that Gladys will be using plasmids in a way that can potentially have a direct impact on human health .... Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer screening ...wrote in her application, “Cervical Cancer is the leading cause of cancer related morbidity and death among...developing countries” and “Almost all cervical cancers are caused by persistent infection of HR HPV types...To start her work, Gladys will improve cervical cancer screening for a limited group of Ghanaian women...

... is our own immune system, which can recognize antigens on cancerous cells and eliminate them from the...Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...suppressors and upregulation of proto-oncogenes ­­­can directly inhibit the ability of a cell to undergo...demonstrated that a number of novel small molecules can bind directly, albeit weakly, to K-Ras and inhibit...list of all available Addgene plasmids he or she can use to study it (as seen in the screenshot below)...Additional Resources National Cancer Institute: Cancer Statistics Page National Cancer Institute: Ras Pathway...tens of millions of new cancer cases arise and millions of people die from cancer-related complications ...

...Argonaute. They identified NgAgo and showed that it can cleave plasmid and genomic DNA in a variety of mammalian...DNA guides may be more user-friendly, since they can be ordered as oligonucleotides and should be stable...transcribed, and changes in gRNA secondary structure can affect editing efficiency. Gao et al. tested if NgAgo...key advantage over Cas9, for which gRNA sequence can greatly affect editing efficiency. Smaller size ... so NgAgo remains faithful to its guide DNA and cannot switch guides. While this fidelity would prevent...