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We narrowed to 928 results for: Gene

Showing: 511 - 520 of 928 results

  1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...during adeno-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475. ...have probably heard about Addgene’s viral service but did you know that Addgene also performs independent...101 eBook Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production...research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ...PMCID: PMC2957237. Additional resources of the Addgene blog Read our beginner's guide to viral vectors...

  2. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...Neddersen characterized a few samples by 16S rRNA gene sequencing which showed the presence of many “dark...Yellowstone National Park (YNP). Extremophiles - phylogenetically diverse microbes living in extreme physical...lab and ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9...particularly useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...

  4. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...China was using CRISPR/Cas9 in an attempt to create gene-edited babies. Several hours later, the scientist...public consensus on genome editing, there was a generally agreed-upon international moratorium on utilizing...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused” in...decisions on scientific, ethical, regulatory, and general public consensus to the next meeting of experts...and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner (in business...on theirpenmanship. Additional resources on the Addgene blog Find all of our CRISPR blog posts Download... genome engineering blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  5. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ... role in the transcriptional regulation of many genes. A number of groups have designed means of studying...with mAID. Using this method, the lab was able to generate conditional alleles of a few essential proteins... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID... MerMAIDs - marine channelrhodopsins for the optogenetic toolbox Article contributed by Susanna Bachle...quickly and with high precision - a valuable optogenetics feature when studying neuronal activity. The...

  6. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though, have...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic...

  7. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... to examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK reporter...Find plasmids from this paper at Addgene. Additional Resources the Addgene Blog Learn More about SunTag... remained elusive. Two techniques developed by Addgene depositors have made it easier to track translation... on the nascent polypeptide recruit scFV-GFP, generating green puncta that colocalize with the red reporter...PMC2746483 Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An...: PMC4451088 Find plasmids from this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation... Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check Out ...

  8. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into your...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...sequence between two target sites. Modifying multiple genes at once. Using multiplexed gRNAs can target multiple...simultaneous multiplex editing of six different genes. This system doesn’t rely on any additional or host-dependent...from this paper at Addgene. 9. Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Browse Our CRISPR Topic...add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express more...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...inhibiting gene expression, detecting neuronal signaling, or manipulating any other genetically controlled...wide array of genetic tools for neural interrogation like optogenetic tools, chemogenetic tools, and biosensors... the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral...injected into the brain and efficiently deliver genetic information or tools, making them useful tracers...efficiently infects neurons, directs high-level transgene expression, and has retrograde functionality to...manipulated (e.g., activated or inhibited) by any genetic tool delivered by the AAV.   AAVrg requires high...evaluating how this serotype works in your system. Chemogenetic vectors  Expression of retrograde serotype vectors...
Showing: 511 - 520 of 928 results