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...increased choice and flexibility; while ensuring we can maintain high standards for both our depositors and...banner on their material page. Interested scientists can expand the banner to select their serotype of choice...’d like, and submit the request. Request details can be accessed within your Addgene account. Preps will...Request prep 6–9 weeks after placing your order.  Can I order Packaged on Request preps through a distributor...packaged DNA and serotype. Please note that Addgene cannot perform functional or in vivo testing. We recommend...

...many years of study to come. Some Freshers Fairs can include fairground rides and other fun activities...between undergraduates and PhD students so that they can help support each other and provide advice. The society... develop a network of fellow scientists that you can grow with, learn from, and mentor while pursuing ...provide strong foundations upon which young scientists can build powerful networks and we encourage them them...

...et al. demonstrate that this new vector, pSiM24, can also be used with biolistic methods, making it useful...controlled by a weaker constitutive Nos promoter, and it can be used to select both transformed Agrobacterium ... are suitable for many applications, the plasmid can also be customized due to its modular structure. ...Given its versatility and customizability, pSiM24 can be used not only for plant engineering and biotechnology...

...Davidson lab for the last four years. “Researchers can make their own target if it is not already made for...backbone with very similar restriction enzymes. You can essentially cut and paste quite quickly. Researchers...included some of their own reference images that can be found on the corresponding plasmid pages (e.g....Maserati. If we know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will...story about his life’s work and the stage IV lung cancer that has now prompted him to deposit the tools ...

...are not aware of what Addgene can do for them and how the repository can help them share their materials...makes her the perfect Addgenie and she can’t wait to see how she can help the scientists in Europe with their...engagement. Information about contacting Addgene can be found on our Contact page. You’ve called. You’...Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology, and genetics ...

...lines. Instead, researchers can generate and maintain transgenic lines that can be used for other experiments...specific protein of interest. This fusion protein can then be co-expressed in essentially any transgenic...designed and optimized with zebrafish in mind, it can easily be applied to other systems.    Find BLITZ...limitations of number of flourescent proteins that can be inserted into a cell without disrupting its functions...

...experiment then you will need to make sure that you can easily distinguish each different type of antibody...antibody and therefore each target protein. This can be accomplished by using a panel of primary antibodies...the assay is a unique isotype or species, then you can choose different fluorophore-conjugated secondary...have a number of options. If this is the case, you can narrow them down in a variety of ways. First, review...and experimental considerations - no other factors can make up for an antibody that doesn't work for your...hundreds of options. With limited time and budget you cannot test them all, so how do you know which ones to...

...transmission in brain slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split...through a host organism, the expression of some genes can change depending on the location. Current methods...given cell type is already known, these reporters can help define the different cells present in a mixed...developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage without affecting...the anticipated effect of the targeted gene. CHIME can also be used for high throughput screens using pooled...

...Tips on drafting a stellar resume and cover letter can be found on the Addgene blog.   3. Personal statement...in doubt, consult the web to find the tools that can help you craft a solid personal statement.  4. Letters...a network of friends, and future colleagues that can help pull one another up while you are climbing the...outcome for the both of you. Next year, your friend can circle back and lend you a hand to get you into that...communities to educate parents about resources that can help their children thrive academically. Rose is ...doors to a web of opportunities that the classroom cannot offer. Furthermore, companies across most industries...Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo...

...our chromosomes is very much 3-dimensional, as you can see by this post’s chromatin illustrations from Leah...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate... plasmids containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives...have many different variant names, not all of which can be listed here. If you don’t see the gene you’re ...of plasmids from the indicated publications. You can request all of these plasmids directly from Addgene...potentially detract from the process being depicted, we can’t forget that those other components are still there...