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We narrowed to 892 results for: CAN

Showing: 521 - 530 of 892 results

  1. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...our chromosomes is very much 3-dimensional, as you can see by this post’s chromatin illustrations from Leah...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate... plasmids containing the indicated gene, and you can subscribe to these pages to learn when Addgene receives...have many different variant names, not all of which can be listed here. If you don’t see the gene you’re ...of plasmids from the indicated publications. You can request all of these plasmids directly from Addgene...potentially detract from the process being depicted, we can’t forget that those other components are still there...

  2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...transmission in brain slices from mouse, and showed that it can alter motor responses in C. elegans. Find the split...through a host organism, the expression of some genes can change depending on the location. Current methods...given cell type is already known, these reporters can help define the different cells present in a mixed...developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage without affecting...the anticipated effect of the targeted gene. CHIME can also be used for high throughput screens using pooled...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...lines. Instead, researchers can generate and maintain transgenic lines that can be used for other experiments...specific protein of interest. This fusion protein can then be co-expressed in essentially any transgenic...designed and optimized with zebrafish in mind, it can easily be applied to other systems.    Find BLITZ...limitations of number of flourescent proteins that can be inserted into a cell without disrupting its functions...

  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...experiment then you will need to make sure that you can easily distinguish each different type of antibody...antibody and therefore each target protein. This can be accomplished by using a panel of primary antibodies...the assay is a unique isotype or species, then you can choose different fluorophore-conjugated secondary...have a number of options. If this is the case, you can narrow them down in a variety of ways. First, review...and experimental considerations - no other factors can make up for an antibody that doesn't work for your...hundreds of options. With limited time and budget you cannot test them all, so how do you know which ones to...

  5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we make a decision...morphological profiling can be used as generalizable strategies to map the function of rare cancer variants and ...functional genomics a reality for future cancer patients. You can follow him on Twitter @boehmjesse.   Additional...Accelerator Plasmid Collection, that we hope researchers can use to characterize these variants in a high-throughput... proven to improve future clinical decisions, we can work together to realize the potential of precision...Collection) or “pan-cancer” analyses across many cancer types (Pan Cancer Mutant Collection). In addition...Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine...

  6. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...Tips on drafting a stellar resume and cover letter can be found on the Addgene blog.   3. Personal statement...in doubt, consult the web to find the tools that can help you craft a solid personal statement.  4. Letters...a network of friends, and future colleagues that can help pull one another up while you are climbing the...outcome for the both of you. Next year, your friend can circle back and lend you a hand to get you into that...communities to educate parents about resources that can help their children thrive academically. Rose is ...doors to a web of opportunities that the classroom cannot offer. Furthermore, companies across most industries...Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo...

  7. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...and it’ll undoubtedly be quite a while before we can firmly gain a handle on understanding and appreciating...perspective, the enzymes obtained from psychrophiles can be used for a diverse array of processes. The food... benefits for any food processing industry as it can potentially help minimize the incidental losses like...suboptimal conditions. This means recombinant proteins can aggregate or precipitate out as insoluble granules...

  8. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ... the hosts, Josh and Dan, discuss how scientists can start using Twitter and provide real-life examples...for more tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...not capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3....do list. This video shows quick ways social media can fit into a researcher’s work day. It’s also important...these links, I’ve: 1) convinced you social media can positively impact your career, 2) given you tips ...Social Media for Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference...

  9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...itself is ~100 bases) limit the number of gRNAs that can be packaged together in a single vector. In addition...protoplasts (15% and 9% of target sites.) How many loci can Cas9 modify? To test the application of this system...frequency at the four loci, indicating that Cas9 can be guided to at least 8 distinct loci. They also ...The use of multiple gRNAs targeting a single gene can increase the likelihood of gene knockout, or multiple... genes with overlapping activity. Multiple gRNAs can also facilitate deletions of other regions, such ...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...three stop codons, meaning that the same amino acid can be encoded by more than one codon. Although the genetic...organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based...certain systems, synonymous changes to a gene sequence can cause unexpected detrimental results to the protein...those that exhibit wobble base-pairing (a tRNA that can recognize multiple synonymous codons) may actually...genes contain a region where a synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall...CDS-located miRNA target sites suggests that they can effectively inhibit translation. Genome Research ...expression limiting regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten...
Showing: 521 - 530 of 892 results