We narrowed to 932 results for: Gene

...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though, have...Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...Pam_Hines and LinkedIn. Additional Resources on the Addgene Blog What Do I Do Now? Academic v. Non-Academic...

...Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into your...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...

...sequence between two target sites. Modifying multiple genes at once. Using multiplexed gRNAs can target multiple...simultaneous multiplex editing of six different genes. This system doesn’t rely on any additional or host-dependent...from this paper at Addgene. 9. Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Browse Our CRISPR Topic...add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express more...

...inhibiting gene expression, detecting neuronal signaling, or manipulating any other genetically controlled...wide array of genetic tools for neural interrogation like optogenetic tools, chemogenetic tools, and biosensors... the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral...injected into the brain and efficiently deliver genetic information or tools, making them useful tracers...efficiently infects neurons, directs high-level transgene expression, and has retrograde functionality to...manipulated (e.g., activated or inhibited) by any genetic tool delivered by the AAV.   AAVrg requires high...evaluating how this serotype works in your system. Chemogenetic vectors  Expression of retrograde serotype vectors...

...Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using CRISPR...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Learn about Photoactivatable...proteins. To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins. Solving the...yield, and when transiently expressed in cells, generates much less signal than comparable fluorescent proteins... approach is especially suited to complement optogenetic tools, where light used for excitation can cause...cause unintended activation of the optogenetic system. Biosensors based on Nano-lantern technology are...

...Technique Q1: Is there any way to deliver a long gene (>4.5 kB) by AAV? A: One potential strategy is to...applications in diseases which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic...an exciting area for future AAV application to epigenetic editing. This area is discussed in our recent...excellent questions.    Additional Resources on the Addgene Blog Using virus in your research - a primer for...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

...as a control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites...coronavirus or COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can .... Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one...reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution to expand... coronavirus related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt...

...that control cell cycle gene expression.  Additional Resources on the Addgene Blog Visit the fluorescent...work in Lin Tian’s lab at UC Davis is to generate a genetically encoded fluorescent sensor for serotonin...sequence an individual’s genome. But when they find genetic variants, what does that mean? Previous research...of Washington sought to address the effects of genetic variants in a high throughput manner. She helped...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...GFP Find microbiology blog posts Resources on Addgene.org Find fluorescent protein biosensors Browse Plasmids...

... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...PP7, or boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination... PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from Ma et al., 2016 ...CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR...PubMed PMID: 30377374. Additional resources on the Addgene blog Download our CRISPR eBook Learn about fluorescent...tools Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR plasmids for visualization...

...telephone, until the message reaches the nucleus where gene expression changes lead to cell proliferation and... on Addgene.org Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics...induced binding. The lab used this general design scheme to generate two more OptoNBs, one against mCherry...loop 1 always generated OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue ...on the key components of OptoNBs, how they are generated, and their use for protein purification and controlling...nanobody’s ability to bind its target protein.  Generating OptoNBs The opening and closing of the AsLOV2...spatiotemporal resolution than pharmacological or genetic manipulation of the signaling pathway.  As a proof-of-principle...