We narrowed to 904 results for: CAN

...variants of unknown significance” (sometimes called “VUS”) function is unknown. How can we make a decision...morphological profiling can be used as generalizable strategies to map the function of rare cancer variants and ...functional genomics a reality for future cancer patients. You can follow him on Twitter @boehmjesse.   Additional...Accelerator Plasmid Collection, that we hope researchers can use to characterize these variants in a high-throughput... proven to improve future clinical decisions, we can work together to realize the potential of precision...Collection) or “pan-cancer” analyses across many cancer types (Pan Cancer Mutant Collection). In addition...Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine...

...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...and it’ll undoubtedly be quite a while before we can firmly gain a handle on understanding and appreciating...perspective, the enzymes obtained from psychrophiles can be used for a diverse array of processes. The food... benefits for any food processing industry as it can potentially help minimize the incidental losses like...suboptimal conditions. This means recombinant proteins can aggregate or precipitate out as insoluble granules...

... the hosts, Josh and Dan, discuss how scientists can start using Twitter and provide real-life examples...for more tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...not capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3....do list. This video shows quick ways social media can fit into a researcher’s work day. It’s also important...these links, I’ve: 1) convinced you social media can positively impact your career, 2) given you tips ...Social Media for Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference...

...itself is ~100 bases) limit the number of gRNAs that can be packaged together in a single vector. In addition...protoplasts (15% and 9% of target sites.) How many loci can Cas9 modify? To test the application of this system...frequency at the four loci, indicating that Cas9 can be guided to at least 8 distinct loci. They also ...The use of multiple gRNAs targeting a single gene can increase the likelihood of gene knockout, or multiple... genes with overlapping activity. Multiple gRNAs can also facilitate deletions of other regions, such ...

... another virus. With the pseudovirus, scientists can study the mechanisms of infection and how to interfere...yields and stability. This stabilized spike protein can be used for the development of vaccines and diagnostics...those encoding different spike protein variants that can be incorporated into pseudoviruses. The lab used ...neutralizing the different SARS-CoV-2 spike variants. You can learn more about their research in this article. ...transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited...MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction to detect ...

...three stop codons, meaning that the same amino acid can be encoded by more than one codon. Although the genetic...organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based...certain systems, synonymous changes to a gene sequence can cause unexpected detrimental results to the protein...those that exhibit wobble base-pairing (a tRNA that can recognize multiple synonymous codons) may actually...genes contain a region where a synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall...CDS-located miRNA target sites suggests that they can effectively inhibit translation. Genome Research ...expression limiting regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten...

...both dCas9 and the gRNA. This expression can vary significantly depending on transgene design and delivery...proteins might not localize to the right places, which can provide misleading information on where exactly a...signal or including fusions with Histone 2A or 2B can result in bright targeted expression while minimizing... found that too high expression of these markers can lead to non-specific labeling. They generated plasmids...be exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for...benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are smaller in size, more...

...Such a behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion can also be used ...an animal running to escape a threat. Locomotion can thus be used as a readout for associated emotional...advantage of these characteristic traits, rodents can be used to study anxiety levels in general, or anxiety...maze that measures risk aversion/anxiety. The mouse can move freely on a 4-arm cross and choose to stay on...recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal species for behavioral...

...read particular studies or experiments. Later, you can easily convert the citation style to whatever the... relevant researchers in the field. Finally, you can sound credible and cool when you casually mention...factorials. In environmental science and ecology, this can be represented by the tip of the iceberg. For writers... like me, you might be surprised at how much you can get done between office hours and experiments. A ...don’t forget to include citations so that people can go back and read the original reference for the data.... Submit your article! Overall, writing a review can be overwhelming and challenging. My best advice is...development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID: 26124919. PubMed...

...managed! The good news is that management skills can be taught, but it will take time so be patient and... learn what motivates them (keep in mind that it can be very different from what motivates you!), know...important is to be able to actively listen - this can be hard - but it is the best way to avoid mistakes...The silence and maybe even the slight awkwardness can spur people to speak up. Once aware of the problem...want to know. Remember that one on one discussion can be confidential. Finally, provide timely feedback...will be grateful for the feedback, but at least you can start making changes for the best. Positive feedback...