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We narrowed to 892 results for: CAN

Showing: 531 - 540 of 892 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...both dCas9 and the gRNA. This expression can vary significantly depending on transgene design and delivery...proteins might not localize to the right places, which can provide misleading information on where exactly a...signal or including fusions with Histone 2A or 2B can result in bright targeted expression while minimizing... found that too high expression of these markers can lead to non-specific labeling. They generated plasmids...be exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for...benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are smaller in size, more...

  2. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...managed! The good news is that management skills can be taught, but it will take time so be patient and... learn what motivates them (keep in mind that it can be very different from what motivates you!), know...important is to be able to actively listen - this can be hard - but it is the best way to avoid mistakes...The silence and maybe even the slight awkwardness can spur people to speak up. Once aware of the problem...want to know. Remember that one on one discussion can be confidential. Finally, provide timely feedback...will be grateful for the feedback, but at least you can start making changes for the best. Positive feedback...

  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...Such a behavior can be monitored in an open field test (Alpar et al., 2018). Locomotion can also be used ...an animal running to escape a threat. Locomotion can thus be used as a readout for associated emotional...advantage of these characteristic traits, rodents can be used to study anxiety levels in general, or anxiety...maze that measures risk aversion/anxiety. The mouse can move freely on a 4-arm cross and choose to stay on...recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal species for behavioral...

  4. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... only the highly restricted number of people who can access the ELN have the ability to attempt to reproduce...kind of informal conversations about research that can take place in Slack normally never become part of...into ELNs would also be useful, so that the data can be viewed – and conceivably additional work relating...relating to reproducing the original research can be documented -- in the original context in which it was...analyzed. While the many different ELNs out there can help with searchability, record-keeping, and data...through interacting with colleagues in Edinburgh. You can follow Rory on Twitter @rory_macneil.     Additional...with a particular experiment or project thus significantly enhances the ability of others to subsequently...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... another virus. With the pseudovirus, scientists can study the mechanisms of infection and how to interfere...yields and stability. This stabilized spike protein can be used for the development of vaccines and diagnostics...those encoding different spike protein variants that can be incorporated into pseudoviruses. The lab used ...neutralizing the different SARS-CoV-2 spike variants. You can learn more about their research in this article. ...transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited...MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction to detect ...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...read particular studies or experiments. Later, you can easily convert the citation style to whatever the... relevant researchers in the field. Finally, you can sound credible and cool when you casually mention...factorials. In environmental science and ecology, this can be represented by the tip of the iceberg. For writers... like me, you might be surprised at how much you can get done between office hours and experiments. A ...don’t forget to include citations so that people can go back and read the original reference for the data.... Submit your article! Overall, writing a review can be overwhelming and challenging. My best advice is...development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID: 26124919. PubMed...

  7. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ... lab has worked extensively with enChIP systems. Can you describe this technology and its advantages? ...enChIP). By using enChIP, specific genomic regions can be easily isolated for identification of associated...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential...involved in these professional activities because I can contribute by, for example, helping to improve the...and Japan (University of Tokyo, Osaka University). Can you speak about your different experiences in research... in Japan, which is very disappointing. I hope I can help change the situation in the future. Addgene:...

  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...internal region of the mRNA. In the figure above, you can see that the IRES element acts as another ribosome... L L K L A G D V E S N P G P * (GSG) residues can be added to the 5' end of the peptide to improve ... the co-expression of multiple unique genes, you can start with a plasmid that has multiple cloning sites...PCR-cloned between your genes of interest and you can then insert the whole multicistronic cassette into... 101 eBook And now for some exciting news... You can now download Addgene's new eBook! Our goal was to...additional resources into one downloadable PDF that you can save on your desktop. The Plasmids 101 eBook is designed...

  9. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...well as their modern equivalents and see what we can learn from their evolution. Southern blotting and..., named for its inventor Edward M. Southern (1), can be used to detect DNA identity, size, and abundance...has a particular gene mutation, southern blotting can be used to identify the presence of that mutated ...bind to the sample thereby labeling it. The label can then be visualized by autoradiography and X-ray film...are also commonly used. In all cases, the result can be quantified by the strength of the signal in comparison...for cloning and analytical digests. A researcher can test the orientation of an insert in only a few hours...nucleotide, a researcher (or a simple computer program) can then reproduce the DNA sequence (Figure 2). Accuracy...

  10. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...more about their work, and how these plasmids can help cancer researchers studying an assortment of genes...population, as mutations with strong phenotypic effects can mask weaker ones. Thus, the need for a faster and...phenotypic effect in a different system. These plasmids can also help scientists understand how a given gene ... genetic perturbations that may play significant roles in cancer pathogenesis. Their work culminated ... from The Cancer Genome Atlas (TCGA), a catalog of genetic mutations responsible for cancer from genomics...interventions for cancer. This screening platform is an incredible boost to the cancer research community...functional annotation of somatic mutations in cancer." Cancer Cell 33.3 (2018): 450-462. PubMed PMID: 29533785...
Showing: 531 - 540 of 892 results