We narrowed to 904 results for: CAN

...three stop codons, meaning that the same amino acid can be encoded by more than one codon. Although the genetic...organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based...certain systems, synonymous changes to a gene sequence can cause unexpected detrimental results to the protein...those that exhibit wobble base-pairing (a tRNA that can recognize multiple synonymous codons) may actually...genes contain a region where a synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall...CDS-located miRNA target sites suggests that they can effectively inhibit translation. Genome Research ...expression limiting regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten...

... lab has worked extensively with enChIP systems. Can you describe this technology and its advantages? ...enChIP). By using enChIP, specific genomic regions can be easily isolated for identification of associated...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential...involved in these professional activities because I can contribute by, for example, helping to improve the...and Japan (University of Tokyo, Osaka University). Can you speak about your different experiences in research... in Japan, which is very disappointing. I hope I can help change the situation in the future. Addgene:...

...internal region of the mRNA. In the figure above, you can see that the IRES element acts as another ribosome... L L K L A G D V E S N P G P * (GSG) residues can be added to the 5' end of the peptide to improve ... the co-expression of multiple unique genes, you can start with a plasmid that has multiple cloning sites...PCR-cloned between your genes of interest and you can then insert the whole multicistronic cassette into... 101 eBook And now for some exciting news... You can now download Addgene's new eBook! Our goal was to...additional resources into one downloadable PDF that you can save on your desktop. The Plasmids 101 eBook is designed...

...this phrase when discussing raising children, it can also be applied to research. Sharing knowledge and...Putative enhancer sequences for specific cell types can be identified based on the wear-and-tear footprint...fluorescent gene and packaged into viral vectors that can be administered systemically (retro-orbitally, RO...resources. Luckily for us, they like sharing! You can find data, analysis tools, and other scientific and... and educational resources on their website. You can find more neuroscience-focused tools in their Brain...this collection of highly specific AAV enhancers can help scientists around the world better understand...understand the brain, how it functions, and how we can help keep it healthy. Thank you to Dr. Yoav Ben-Simon ...

...well as their modern equivalents and see what we can learn from their evolution. Southern blotting and..., named for its inventor Edward M. Southern (1), can be used to detect DNA identity, size, and abundance...has a particular gene mutation, southern blotting can be used to identify the presence of that mutated ...bind to the sample thereby labeling it. The label can then be visualized by autoradiography and X-ray film...are also commonly used. In all cases, the result can be quantified by the strength of the signal in comparison...for cloning and analytical digests. A researcher can test the orientation of an insert in only a few hours...nucleotide, a researcher (or a simple computer program) can then reproduce the DNA sequence (Figure 2). Accuracy...

...A viral vector that can target specific tissues, even when administered systemically, without causing...Recombinant adeno-associated viral vectors, or rAAVs, can sound almost too good to be true! In a previous post...genetic material, and variations between serotypes can change how the viral particle sneaks its way into...multiple tissues, rapid transgene expression, and can cross blood vessel barriers. It is especially popular... targeting cardiac cells (Bish et al., 2008). It can also cross the blood-brain barrier, making it useful...receptor published this year (Dhungel et al., 2025)! It can transduce neurons for retrograde labeling, as well...addition to tissue tropism, different AAV serotypes can be useful to evade neutralizing antibodies developed...

...mismatches that can nevertheless still lead to activity has been established, and can be used to identify...gRNAs. A more extended discussion of these subjects can be found in two recent review articles (Doench et...importance (target should be near the TSS but you can worry less about sequence optimality because you ...variants thereof offers additional PAM options that can greatly expand gRNA options. Created with ... in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive score, as well...computational prediction is ever perfect, but this can decrease the number of guides one needs to test in...minimizing off-target activity, which sounds obvious but can often require difficult decisions. For example, would...

...kinds of problems can, of course, be solved with good management practices, but can sometimes go unnoticed...those contracts fall through or are cancelled, then redundancies can be made within the company; new staff...large company (both for-profit and non-profit) you can also get a lot of office politics (many individuals...projects and away from other individuals projects, this can cause conflicts and favoritism, as well as affect...have less work to do than was anticipated and there can be a glut of individuals with similar skill sets....was entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia or... my job. Because of my background in genetics, I can help scientists problem solve experiments over the...

...tuned for part two of Caitlin’s interview where you can learn what it’s like to work for a science education...

...Perhaps there are examples of science writers who can get everything that they need just from reading up...