We narrowed to 904 results for: CAN

...more about their work, and how these plasmids can help cancer researchers studying an assortment of genes...population, as mutations with strong phenotypic effects can mask weaker ones. Thus, the need for a faster and...phenotypic effect in a different system. These plasmids can also help scientists understand how a given gene ... genetic perturbations that may play significant roles in cancer pathogenesis. Their work culminated ... from The Cancer Genome Atlas (TCGA), a catalog of genetic mutations responsible for cancer from genomics...interventions for cancer. This screening platform is an incredible boost to the cancer research community...functional annotation of somatic mutations in cancer." Cancer Cell 33.3 (2018): 450-462. PubMed PMID: 29533785...

..., high intensity exposure can cause DNA damage. Far-red light, however, can penetrate 5 mm past the surface...CRISPR system. This common optogenetic technique can be employed in other split systems where light is...2015) resulting in a double strand break (DSB) that can be repaired by either non-homologous end joining ...Single-chain Cas9 photoswitches Photoactivation of Cas9 can also be achieved using single-chain fusion proteins... of AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was...Hoffman et al., 2020). Fluorescent dCas9 proteins can be used to identify specific genomic loci for imaging...imaging techniques. Since expression of dCas9 can potentially alter the genome and chromatin architecture...

...Cpf1 can process its own pre-crRNA arrays. Zetsche et al demonstrate this by showing that Cpf1 can cleave... to express the gRNAs and Cas9. Co-transfections can lead to variable expression levels due to differences...is a suitable substrate for Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging...repeats, leading to expression of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was ...

...SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then be used...learn more about it? Read on to find out how ccdB can make your cloning experiments a little easier. One...ccdB alone isn’t the only key to the system – how can one work with a plasmid/gene that kills the cells...function, there is some evidence that ccdB Survival™ can be more difficult to transform, depending upon the...

... role in the severity of breast cancer. Infectious Disease. RaPID can also help identify which host proteins...naturally occurring complexes present in the cell, it can also introduce non-physiological RNA-protein interactions...like a nifty technique, but what kinds of questions can it help answer? Ramanathan et al. outline three applications...to Zika replication. Human genetic disease. RaPID can also be used to test how RNA variants associated ...patients. These are just three examples of how RaPID can be used to study RNA-protein interactions. Do you...in breast cancer patients. By digging into the literature and analyzing data from The Cancer Genome Atlas...other BirA*-based proximity labeling methods, RaPID cannot distinguish between proteins that directly or indirectly...

...used for targeting; however, these options can be cumbersome, can lower on-target efficiency, and sometimes...similar mutations can improve the specificity of Cas9 derived from S. aureus. This means you can likely apply...It is well known that CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means...

.... Thus, T-DDOGs can be used in combination with green-red Cre-lox systems. T-DDOGs can also be designed...T-DOGGs can be made drug-inducible using the rTetR system, but Cre-DOG lacks the toxicity that can be seen...their system, Tang et al. found that YFP and CFP can activate certain T-DDOG constructs similarly to GFP...

...double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target editing. In this...both strands bluntly with WT Cas9 and one gRNA, you can create a staggered cut using a Cas9 nickase and two...target different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation...20% repair efficiency. Clearly, a nickase strategy can expand the targetable region, which is especially...site. So if you can’t find a good gRNA that cuts close to your insertion site, you can’t obtain high HDR...

...had success with significantly fewer wings, such that enough tissue for an experiment can be easily collected...obtaining enough material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  ...short incubation at 37 °C. These short DNA fragments can then be purified for subsequent library preparation...science.     Additional Resources Learn how you can use FRET to study protein interactions Learn about...Washington. CUT&RUN doesn’t require fixation and significantly decreases background noise, reducing the read...improvements to the initial CUT&RUN protocol, the most significant being the usage of a strong detergent to permeabilize...extraction. They report that this change offers a significant improvement and simplification of the original...

...more precisely control the amount of labeling, you can alter the attP site. Truncating the attP site upstream...Applications for SPARC SPARC technology is versatile and can be applied across cell types and organisms by replacing... the construct. In Drosophila, the SPARC systems can be used both for visualization and for optogenetics...of fly cell types. Because the PhiC31 recombinase can work in other model organisms including mouse and...