..., high intensity exposure can cause DNA damage. Far-red light, however, can penetrate 5 mm past the surface...CRISPR system. This common optogenetic technique can be employed in other split systems where light is...2015) resulting in a double strand break (DSB) that can be repaired by either non-homologous end joining ...Single-chain Cas9 photoswitches
Photoactivation of Cas9 can also be achieved using single-chain fusion proteins... of AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was...Hoffman et al., 2020). Fluorescent dCas9 proteins can be used to identify specific genomic loci for imaging...imaging techniques. Since expression of dCas9 can potentially alter the genome and chromatin architecture...