We narrowed to 792 results for: KIN

1. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ... noise in the process of sampling and inference-making. Even perfect transparent and rigorous studies ...significant result that may be the result of noise, p-hacking, or unclear methods. Many thanks to our guest ...

2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot...signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs...

3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...efficiency. Check out Addgene’s blog to learn about making your own competent cells and our protocols page...passed down through cell division. You can find all kinds of different lentiviral and AAV plasmids as well...

4. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ...however can interfere with other genetic pathways making it difficult to link a phenotype to the gene being...TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo podcast segment...

5. Clear and Accessible Writing

   Type

   Blog Post

   Published

   Feb. 15, 2024, 2:15 p.m.

   ...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the...helpfully. You and your readers are now a team, working together to share information. Sometimes it is ...

6. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et al....

7. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...FRET-based sensors such as Twitch. While calcium is king in the sensor world, there are sensors available...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...

8. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ...replicate and spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue...viral applications to help you start (safely!) thinking about the possibilities. Ready to plan your next...

9. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...behind. While commercial kits are available for making small point mutations, large deletions/insertions...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage...