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We narrowed to 892 results for: CAN

Showing: 561 - 570 of 892 results

  1. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ...’s progress in international distribution As you can see from the chart below, we are making great progress...Instrumental Access equipment from Seeding Labs and that can demonstrate that these plasmids will effectively ...like it. Additional Resources Find out how you can get involved with Seeding Labs Follow Seeding Labs...challenges. However, these same researchers face significant barriers to scientific discovery and career development...

  2. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...fascinating to me was the outdoor market where one can shop for components of traditional Chinese medicine...replication. He too mentioned how “surprising” small RNAs can be and posited that “we underestimate living things...They have created a circRNA website so scientists can view public circRNA datasets and download the custom...to discover circRNAs in RNA-seq data. As far as I can tell, we don’t really know what circRNAs do yet or... able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015 conference invited... of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne Kamens, PhD...

  3. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...resource for bench scientists everywhere. In it, you can find an introduction to viral vectors and their elements...content in a clean and easy-to-navigate design. You can find it, along with other eBooks in our collection...

  4. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...Understanding the different types of CRISPR systems can be as confusing as reading Shakespeare for the first...CRISPR systems are less popular in the lab. This can be attributed to the multi-Cas effector, Cascade....power of Class 1 systems. Class 1 CRISPR systems can be further broken down into type I, type III, and...CRISPR systems. Type III systems are unique, as they can technically target both RNA and DNA, though it is...that they are involved in plasmid competition or can hijack machinery from other CRISPR systems to carry...people are familiar with. Class 2 CRISPR systems can be further broken down into type II, type V, and ...alternatives to Cas9. Cas12 is unique because it can process multiple gRNAs under a single promoter, allowing...

  5. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...these multi-component systems can be complicated to implement, they can be highly specific with a small...comfortable with it or can find a colleague to help you, synthesizing your own ligands can open up more possibilities...more). So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures or low levels...the same imaging workflows you already use. Or you can really capitalize on the dye’s performance and use...’re working with purified proteins in vitro, you can even use the same tag for multiple proteins but label...limited to fluorescent ligands! Self-labeling tags can also be used for surface immobilization, purification...

  6. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...yielding 1-10% knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating...process takes ~8-10 months. With CRISPR (B), you can inject CRISPR components directly into a zygote and...repair, with researchers hypothesizing that they can efficiently integrate into the DNA through an HDR...sequence or place it far from the PAM; if not, you can add a silent PAM or guide mutation. Designing an ... How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template!...insertion feature (e.g., a fluorescent protein), you can amplify the desired region using primers containing...the ssDNA construct as described in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein....

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...transfected it into your target cells, now what? How can you tell if the protein you are so keen to study ...detect the presence or absence of a protein, but can also determine if proteins are being up- or downregulated...standards, detect the cellular location of proteins, and can be a readout for protein interaction studies such...standard of known molecular weight in a separate lane can help confirm the size of the protein of interest....high binding capacity. In addition, PVDF membranes can be repeatedly stripped of an antibody and reprobed...plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your search, first pay...useful readout for a number of cellular assays. They can determine protein interactions following immunoprecipitation...

  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... aptamer? RNA is not inherently fluorescent and can be challenging to track intracellularly, but aptamers...to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative, but not quantitative...expression of coding RNA. Spinach-based riboswitches can be used as stable metabolite sensors for imaging ...developed in a study of artificial guide RNAs that can be used to simultaneously target cellular RNA to ...Broccoli has been developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer...observed when these RNAs complex and the entire aptamer can hybridize and fold. (Alam et al., 2017). Yellow fluorescent...selection and directed evolution." Journal of the American Chemical Society 136.46 (2014): 16299-16308. PubMed...

  9. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...presentations and interviews. But how can I adapt this setup for the lab, so we can get back to producing protocol...for free. We’re a small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer...on-screen, somebody who really knows the protocol and can guide viewers through. I know cameras, but we needed...the scenes footage in the last several years, you can usually see a director sitting in their chair not...some more important lab work from being done. You can watch the first video in our Intro to the Lab Bench...

  10. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...school has its ups and downs for everyone but you can come out on top if you leverage your community, think...'s) thesis defense. This wonderful piece of work can be found here: https://www.youtube.com/watch?v=lQGAsgjjBDI...post-doc project 19:55 - 22:40 : Improvements that can be made to the grad school process 22:41 - END : ...beyond graduate school. Ben J. Vincent is a Ph.D candidate in the Program in Genetics and Genomics at Harvard...
Showing: 561 - 570 of 892 results