We narrowed to 904 results for: CAN

...responsibility in our lab’s environmental impact, but we can all do more to make research more environmentally...will likely end up in a landfill, but at least we can reduce the rate at which that happens and reduce ...t offer plastic recycling, specialized companies can recycle plastic-- you just have to find them (or ...commonly accepted at recycling facilities. Your lab can buy a box with the prepaid label and fill it with...

...Rosella can be used to track the targeted autophagy of mitochondria, or mitophagy. Rosella can also be...autolysosomes, this ratio is high. When untargeted, Keima can be used to measure bulk autophagy, but the addition...degradation by autophagy. The RFP-LC3∆G, however, can not be conjugated to PE due to a C-terminal glycine...fluorescent relies on lysosomal acidity, this biosensor can’t be used with fixed cells. Find the mitochondria-targeted...

...scientists with new tools and protocols that they can easily incorporate into their own labs. This week...submission of the results of our first study which can be accessed here at bioRxiv. AAV2 interacts with ...that this effect is not specific to sucrose, but can be adapted to other sugars such as sorbitol. As sorbitol...excited? For starters, we believe that this approach can be adapted by vector cores and laboratories alike...viral particle’s affinity for heparan sulfate proteoglycans on the surface of the packaging cells (Vandenberghe...

...descriptions sound interesting? Is the work something you can see yourself enjoying?  To find master’s-level postings...advancement and growth? A master’s is a tool that can help you advance your career, promote career growth...job title doesn’t change. Master’s level training can prepare you to present your work at conferences, ...decision, but hopefully the questions in this blog post can help you start to figure out if a master’s is a good...looking for PhD applicants since many will also accept applications from master’s candidates with experience...

...plasmids articles. Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral...Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form...tool for researchers to control K+ gradients and can be used as a potent optogenetic silencer. The current... is a string of 10 amino acids, EQKLISEEDL, that can be incorporated into your protein of interest and...

...year. While we love academic research, we hope we can help all researchers achieve happiness in their careers...editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations...gives a quick rundown of some of the strains you can use to express your protein of interest. 4. Plasmids...Non-homologous End Joining So you know that CRISPR can be used to generate knockouts, but how does it work...

...screens in naïve T cells. More broadly, this system can be used to perform in vivo screens in other cell ...introduction of unique molecular identifiers, which can improve the statistical power of in vivo screens....responses in tumors, demonstrating that this system can also reveal novel pathways. The FITS deposit also...regulators of immunity. However, in vitro screens cannot faithfully model the complex spatial, cellular,...putatively involved in CD8+ T cell responses to cancer. The screen recovered known regulators, such as...interested in improving CD8+ T cell responses to cancer.   References and resources Milling, L. E., Markson...Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System: Deciphering the Relationship...

...Can you imagine how much space a million plasmids would fill? Two weeks ago, Addgene edged passed our...we will be moving into a bigger space so that we can make an even bigger impact - we are always on the...facilitate and accelerate scientific discovery. How can Addgene help accelerate your research? Let us know...

...’s progress in international distribution As you can see from the chart below, we are making great progress...Instrumental Access equipment from Seeding Labs and that can demonstrate that these plasmids will effectively ...like it. Additional Resources Find out how you can get involved with Seeding Labs Follow Seeding Labs...challenges. However, these same researchers face significant barriers to scientific discovery and career development...

...fascinating to me was the outdoor market where one can shop for components of traditional Chinese medicine...replication. He too mentioned how “surprising” small RNAs can be and posited that “we underestimate living things...They have created a circRNA website so scientists can view public circRNA datasets and download the custom...to discover circRNAs in RNA-seq data. As far as I can tell, we don’t really know what circRNAs do yet or... able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015 conference invited... of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne Kamens, PhD...