We narrowed to 790 results for: Cre
-
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...
-
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive two-component...pathways. They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....leadership roles leads to more ideas, initiative and creative thinking. The team members will be more invested...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction...500bp to 10kb, with the 3.0kb fragment having increased intensity to serve as a reference band. The uncut... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m.... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E HIST1H2BB...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog PostPublishedJuly 6, 2017, 1:59 p.m....Immuno-Oncology”) is exploding. Researchers are increasingly able to harness the body’s immune system to ...microenvironment Tumor Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....the number of fluorescent proteins is steadily increasing. Often, the choice for a fluorescent protein ...of a fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness...